I generally use CL Sturkey Extremus Low Profile Blades for pretty much everything...when I am having trouble with intestine sections, I use the DuraEdge Encore blade and it usually works very well.
Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, April 23, 2013 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Happy Lab Week from Pam Barker and RELIA Solutions!! (Ian R Bernard) 2. staining near pap pen only???? (Emily Sours) 3. Re: staining near pap pen only???? (Emily Sours) 4. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Sue Hunter) 5. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Vanessa Perez) 6. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Vanessa Perez) 7. RE: Direct Immunofluorescence on Benchmark XT/Ultra (Vanessa Perez) 8. Forwarded post for a histonet member: Subject: CLIA 24-hour gross review. (Sandy Cope-yokoyama) 9. RE: Blades (McAnn, Sherrian) 10. New Lab (Hale, Meredith) ---------------------------------------------------------------------- Message: 1 Date: Mon, 22 Apr 2013 17:22:13 +0000 From: Ian R Bernard <ibern...@uab.edu> Subject: RE: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! To: Pam Barker <rel...@earthlink.net>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <d4f4c602b10b9f45b4e9271af6380e16181b7...@uabexmb1.ad.uab.edu> Content-Type: text/plain; charset="iso-8859-1" - We are celebrating with sponsor breakfasts and lunches for lab personnel from local restaurants each day. - Different internal games. - Educational Lab Week information and mementos for patients and staff alike - And finally, two half days this week, some personnel will go hiking and others on another day horseback riding. That way our mission is staffed without negative impact to patient care V/r Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Monday, April 22, 2013 11:49 AM To: Histonet Subject: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! Hi Histonetters. Happy Lab Week!! Wow this year is just flying by!! It was just Histotechnology Professionals Day a short time ago and here it is lab week already! So here is my question. How is your facility celebrating Lab Week? I hope you are gearing up for a fun week and if you need some ideas for lab week check out Advance Magazine, they have some fun ideas. I think we both know it wouldn't be an email from me if I didn't tell you about my current openings. Please take a second and check them out. Here Are My Spotlight Opportunities: Night Shift Histotech - Patterson, NJ Brand New Lab!! Day Shift Histotech - Salem, VA beautiful area and great Team HT/HTL Mohs Histotech - Long Beach, CA - Make it your own brand new in office lab! The rest of the histology positions that I am most excited about are located in these areas: Nashville, TN Waco, TX Tyler, TX -2 sites one at a hospital and one at a private dermpath lab Atlanta, GA Charlotte, NC Staunton, VA Louisville, KY Staunton, VA Remember if you refer someone to me and I place them now or in the future You will earn a 500 dollar referral fee!! If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. I can be reached at 866-607-3542 or rel...@earthlink.net?Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 22 Apr 2013 13:23:46 -0400 From: Emily Sours <talulahg...@gmail.com> Subject: [Histonet] staining near pap pen only???? To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <CAP=XX1x-xixxbVPHWoKc8HTe9-v=gmLSe+tAp7Obt8mGo=y...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hello Histonetters! We have been doing triple staining on frozen tissue for a while. Just recently it started to stain only near the pap pen (the sections have two lines of pap pen, one where they begin and one where they end). This makes no sense to me, as the staining isn't only on one end (as if the tray was crooked) but both ends and NOT the middle. Can anyone think of what would cause this? My boss came up with the idea that the solution is evaporating and therefore, it's more concentrated on the ends, but that seems a stretch. Then again, it's better than what I came up with, which is nothing! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" ------------------------------ Message: 3 Date: Mon, 22 Apr 2013 13:35:48 -0400 From: Emily Sours <talulahg...@gmail.com> Subject: Re: [Histonet] staining near pap pen only???? To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <CAP=XX1xbGqW4OD-EXdR=h1yc+XvM9j5H1=vx9ahbeljcjza...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Sorry, I forgot to mention, this is manual staining. Also, the area between the pap pen appears to be covered evenly with solution. "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Mon, Apr 22, 2013 at 1:32 PM, Morken, Timothy < timothy.mor...@ucsfmedctr.org> wrote: > Emily, It could be that the reagent is pooling off the ends of the tissue. > Have you observed the tissue during staining to see what is happening > to the liquid on the slide? Is this manual or automated? > > I agree it is strange, because the usual artifact you see with the pap > pen is that the pen liquid covers part of the tissue and THAT part > does not stain. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours > Sent: Monday, April 22, 2013 10:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] staining near pap pen only???? > > Hello Histonetters! > > We have been doing triple staining on frozen tissue for a while. Just > recently it started to stain only near the pap pen (the sections have > two lines of pap pen, one where they begin and one where they end). > This makes no sense to me, as the staining isn't only on one end (as > if the tray was > crooked) but both ends and NOT the middle. Can anyone think of what > would cause this? > My boss came up with the idea that the solution is evaporating and > therefore, it's more concentrated on the ends, but that seems a stretch. > Then again, it's better than what I came up with, which is nothing! > > Emily > > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a > new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Mon, 22 Apr 2013 19:29:56 +0000 From: Sue Hunter <shun...@beaumont.edu> Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra To: Mel John del Barrio <meljdelbar...@yahoo.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <b493b3e4ef638a41875845cedf938b440156a...@exmail04.ms.beaumont.edu> Content-Type: text/plain; charset="iso-8859-1" We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 22 Apr 2013 14:40:39 -0500 From: Vanessa Perez <vpe...@pathreflab.com> Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c80b5ee351aad74dadab70fabcb78d193bfeff7...@exchange.pasa.local> Content-Type: text/plain; charset="iso-8859-1" We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 22 Apr 2013 15:22:29 -0500 From: Vanessa Perez <vpe...@pathreflab.com> Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra To: "eloner...@metrocast.net" <eloner...@metrocast.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c80b5ee351aad74dadab70fabcb78d193bfeff7...@exchange.pasa.local> Content-Type: text/plain; charset="iso-8859-1" Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together. We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips.... Vanessa -----Original Message----- From: eloner...@metrocast.net [mailto:eloner...@metrocast.net] Sent: Monday, April 22, 2013 3:10 PM To: Vanessa Perez Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates. Sent via BlackBerry by AT&T -----Original Message----- From: Vanessa Perez <vpe...@pathreflab.com> Sender: histonet-boun...@lists.utsouthwestern.edu Date: Mon, 22 Apr 2013 14:40:39 To: histonet@lists.utsouthwestern.edu<histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 23 Apr 2013 06:49:39 -0500 From: Vanessa Perez <vpe...@pathreflab.com> Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra To: "Truscott, Tom" <ttrus...@vetmed.wsu.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c80b5ee351aad74dadab70fabcb78d193bfeff7...@exchange.pasa.local> Content-Type: text/plain; charset="iso-8859-1" You just need the FITC antibodies. No kit required. The protocol is under the procedure Fluorescence IHC Basically all the machine does is dispense the antibody on the slide for however long you choose. And some rinses of reaction buffer... Vanessa -----Original Message----- From: Truscott, Tom [mailto:ttrus...@vetmed.wsu.edu] Sent: Monday, April 22, 2013 3:52 PM To: Vanessa Perez Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi Vanessa, I've been following this line of email, since I may need to start doing fluorescence on XT. Which kit/program/protocol do you use on the XT? Can you run them without a kit? Thankyou, Tom T -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Monday, April 22, 2013 1:22 PM To: eloner...@metrocast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together. We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips.... Vanessa -----Original Message----- From: eloner...@metrocast.net [mailto:eloner...@metrocast.net] Sent: Monday, April 22, 2013 3:10 PM To: Vanessa Perez Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates. Sent via BlackBerry by AT&T -----Original Message----- From: Vanessa Perez <vpe...@pathreflab.com> Sender: histonet-boun...@lists.utsouthwestern.edu Date: Mon, 22 Apr 2013 14:40:39 To: histonet@lists.utsouthwestern.edu<histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these "solutions" is an acceptable one for us, we still do them the "old"way and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion..... Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi? All ? Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems? you have encountered? ? ? Thanks ? MJ ? ? Image by FlamingText.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 23 Apr 2013 13:12:26 +0000 From: Sandy Cope-yokoyama <sandy.cope-yokoy...@childrens.com> Subject: [Histonet] Forwarded post for a histonet member: Subject: CLIA 24-hour gross review. To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <6f81318c8f5f984d972778b918a7d769310...@cmcpbexmail12.childrens.med> Content-Type: text/plain; charset="us-ascii" I am the sole histologist in a gastroenterology laboratory situated in a GI clinic. Complying with the 24-hour grossing review by the pathologist is a challenge because my slides travel by courier across town to the pathologist to read. I receive specimens throughout the day and perform grossing in the early AM then again at 11 AM. My pathologist signs copies of the requisition that I fax over that contains the gross and sends them back to me. On a daily basis this works just fine. Fridays and holidays are a pickle but we manage. My question is the intent of the 24-hour review. The inspector cannot tell me why this was put in place, what the pathologist is supposed to do with this information and/or how to tell if it is accurate because he does not receive the slides until the following day which would be outside of the 24-hour review rule. Can anyone tell the intent of the rule? How does this rule improve patient care? Even in a hospital laboratory, once a biopsy is grossed and placed in a cassette, how on earth does looking at the written gross of how big the specimen is or what color it is impact further actions that can take place? What remedial action does CLIA expect? Thanks for your input, Gastro Gal Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ------------------------------ Message: 9 Date: Tue, 23 Apr 2013 11:42:43 -0500 From: "McAnn, Sherrian" <sherrian.mc...@va.gov> Subject: RE: [Histonet] Blades To: "Hale, Meredith" <mh...@miracals.com>, <Histonet@lists.utsouthwestern.edu> Message-ID: <61e2b58cecef384094a363989d47c09009ba2...@vhav17msga2.v17.med.va.gov> Content-Type: text/plain; charset="us-ascii" I have been in histology and cutting for about 26 years now. I have used many types of blades, high and low profile. My favorite and I think the best ones are Surgipath Teflon coated high or low profile blades. I believe the high profile blades are the best but I do realize that not everyone has that option. I think if you tried them you wouldn't be disappointed. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hale, Meredith Sent: Monday, April 15, 2013 12:39 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks ! Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.com<mailto:mh...@miracals.com<mailto:mh...@miracals.com%3 cmailto:mh...@miracals.com>> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 23 Apr 2013 16:50:27 +0000 From: "Hale, Meredith" <mh...@miracals.com> Subject: [Histonet] New Lab To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <0E828EC51C7CC445A51E53F81B64E8C722D557@s-irv-exchmb.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems" Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.com<mailto:mh...@miracals.com> Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.com<mailto:mh...@miracals.com<mailto:mh...@miracals.com%3cmailto:mh...@miracals.com>> ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 24 ***************************************** This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet