Hi Jason, I am not familiar with the stainer but is the procedure starting from water (not dewaxing & re-hydration as is used for paraffin sections)?
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Renz, Jason Sent: Wednesday, 15 May 2013 5:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone marrow aspirate iron stain Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions? Jason Renz HT(ASCP)/lab safety officer St. Alexius Medical Center 900 East Broadway Bismarck ND, 58506 (701)530-6733 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
