I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The tissues have been air-dried but not baked. I cannot obtain more slides.
I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on. We have run slides like this in the past with mixed results. Most of the time, the tissue washes off. Any tips or tricks that might help out in this situation? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
