Her 2 Antibody I do Manual IHC with Tissue Tek Slide Racks and have the same problem. I think the problem is in the holder clip. Only the top sample stains well. Cesar Romero Buenos Aires Argentina > From: histonet-requ...@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 115, Issue 11 > To: histonet@lists.utsouthwestern.edu > Date: Tue, 11 Jun 2013 10:00:54 -0700 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: samples (Webb, Dorothy L) > 2. Re: Histonet Digest, Vol 115, Issue 10 (Wanda Shotsberger Gray) > 3. RE: bone marrow aspirations (Cathy) > 4. Her-2 antibody (Robert Fauck [CCDHB]) > 5. Heinz body stain on already dried blood smears. > (Jackman, Mary Ione) > 6. Telepathology (Gloria Tharp) > 7. RE: Telepathology (Elizabeth Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 10 Jun 2013 12:24:04 -0500 > From: "Webb, Dorothy L" <dorothy.l.w...@healthpartners.com> > Subject: [Histonet] RE: samples > To: "'sa...@royalmarker.com'" <sa...@royalmarker.com> > Cc: "'histonet@lists.utsouthwestern.edu'" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <65365f35c0f2ef4d846ec3ca73e49c4302758cf69...@hpemx3.healthpartners.int> > > Content-Type: text/plain; charset="utf-8" > > It is actually not a website but the internet communication (like a > listserve) they have for PA’s. For instance, histology has one at > histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> > that is used to communicate and network online. > > Thanks again! > > Dorothy Webb > > From: sa...@royalmarker.com [mailto:sa...@royalmarker.com] > Sent: Monday, June 10, 2013 12:20 PM > To: Webb, Dorothy L > Subject: RE: samples > > No problem, the samples are on the way. How were you recommended for our > product? Could you let us know which website you are referring to? > > Thanks Again, > Shawn Jenkins > > > -------- Original Message -------- > Subject: RE: samples > From: "Webb, Dorothy L" > <dorothy.l.w...@healthpartners.com<mailto:dorothy.l.w...@healthpartners.com>> > Date: Fri, June 07, 2013 1:40 pm > To: "'sa...@royalmarker.com<mailto:sa...@royalmarker.com>'" > <sa...@royalmarker.com<mailto:sa...@royalmarker.com>> > Thank you very much. Your marking inks were recommended on the website for > PA’s. > > Your efficient response is greatly appreciated and we will get back to you > next week with feedback! > > Dorothy Webb > > From: sa...@royalmarker.com<mailto:sa...@royalmarker.com> > [mailto:sa...@royalmarker.com] > Sent: Friday, June 07, 2013 12:30 PM > To: Webb, Dorothy L > Subject: RE: samples > > Hello Dorothy, > > We will gladly send you a sample pack of our marking dyes to try. It will > ship out today via UPS to the address below that you provided. I'll be sure > to have your name as well as Jesse Spates on the label as well for a contact. > The set will include 10 1/4oz bottles (all of our colors). Please contact us > with any comments on the product. We appreciate your feedback and hope you > are happy with our products. Have a great weekend and look forward to hearing > from you. > > Best Regards, > Shawn Jenkins > Royal Marker > (P) 954-462-0264 > (F) 954-374-7290 > > > -------- Original Message -------- > Subject: samples > From: "Webb, Dorothy L" > <dorothy.l.w...@healthpartners.com<mailto:dorothy.l.w...@healthpartners.com>> > Date: Fri, June 07, 2013 11:59 am > To: "'sa...@royalmarker.com<mailto:sa...@royalmarker.com>'" > <sa...@royalmarker.com<mailto:sa...@royalmarker.com>> > Cc: "Spates, Jesse D" > <jesse.d.spa...@healthpartners.com<mailto:jesse.d.spa...@healthpartners.com>> > Our PA’s are looking to try other inks than what we are currently using so we > are wondering if we could please get some ink samples. > > Our hospital is Regions Hospital > 640 Jackson St. > St. Paul, MN. 55101 > > I am the technical specialist of histology, Dorothy Webb and the lead PA is > Jesse Spates @ 651-254-4913. > > Thank you, > > Dorothy Webb > Regions Histology Technical Specialist > 651-254-2962 > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this communication in error, please return it to the > sender immediately and delete the original message and any copy of it from > your computer system. If you have any questions concerning this message, > please contact the sender. Disclaimer R001.0 > > ------------------------------ > > Message: 2 > Date: Mon, 10 Jun 2013 18:00:12 -0400 > From: Wanda Shotsberger Gray <wgra...@sc.rr.com> > Subject: [Histonet] Re: Histonet Digest, Vol 115, Issue 10 > To: histonet@lists.utsouthwestern.edu > Message-ID: <qyl2qk6jorpq0tcykafkgfyo.1370901612...@email.android.com> > Content-Type: text/plain; charset=utf-8 > > Hi Megan, > Welcome to the anatomic side! I just googled "history of tissue dyes" and a > wealth of information popped up including this PDF: > www.immunologie-labor.com/cellmarker_files/IET_reagents_07.pdf > all about the discovery of hematoxylin and aniline dyes. The time period you > mentioned was the time of these discoveries, so searching for history of > hematoxylin or eosin should also get you good hits. Good luck! > Wanda Shotsberger > HT/HTL (ASCP) > > ------------------------------ > > Message: 3 > Date: Mon, 10 Jun 2013 17:03:14 -0700 > From: "Cathy" <cf...@007group.com> > Subject: RE: [Histonet] bone marrow aspirations > To: "'Shea's'" <jshea...@roadrunner.com>, > <histonet@lists.utsouthwestern.edu> > Message-ID: <001b01ce6637$11eb70c0$35c25240$@007group.com> > Content-Type: text/plain; charset="us-ascii" > > We are using a similar method at our site. The aspirate is placed into EDTA > and then into formalin to fix. We pour the aspirate through a biopsy bag, > open the bag and scrape the spicules together, transfer them to another > biopsy bag and process as usual. I have received several compliments on the > quality of the slides. > > Cathy > Kelowna, B.C. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's > Sent: Saturday, June 1, 2013 4:26 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] bone marrow aspirations > > I am also interested in how others are processing bone marrow aspirates. > Currently, we let the aspirate clot on a watch glass for about 20 minutes, > gentle slide it onto filter paper, roll it around to get rid of the excess > fluid, place the clot between sponges, and process as usual. > However, I am interested in knowing if any one out there is using a > technique that we use to use at another hospital 33 years ago. > If I remember correctly, the pathologist or oncologist would collect the > aspirate using a heparin syringe. Some how, the aspirate smears for > Hematology were made on 22x22 cover slips instead of slides. Then the EDTA > tube was then given to Histology. I can't remember if we added formalin or > B5 fixative to it at that time, but it was filtered into an obex tea bag > type filter paper (The blood never clotted and ran through the filter paper, > leaving only bone marrow spicules in the paper). We would fold the paper, > place it in the cassette, and process as usual. The pathologist preferred > this method because he wouldn't have to look at levels of clot to find > spicules. Is there anyone out there using this method? > Jan > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Tue, 11 Jun 2013 17:04:35 +1200 > From: "Robert Fauck [CCDHB]" <robert.fa...@ccdhb.org.nz> > Subject: [Histonet] Her-2 antibody > To: histonet@lists.utsouthwestern.edu > Message-ID: > <9c92dfb6ea9983408aa9785182e9fc96680...@wn0nteml11.ad.ccdhb.health.nz> > Content-Type: text/plain; charset=us-ascii > > Hi All > > We here in wellington Hospital New Zealand having some problem with the > Her-2 ( Lieca, NCL-L-CBE-356 ) > > Top half of the slide ( control or Test tissue) are strongly positive > but if we put the control tissue on the bottom half it is week to > negative! > > Does any one has the same problems with your Her-2, it is done on a > Leica Bond (Max) III. > > Much appreciated for any suggestions, also from Leica Technical > specialists. > > Regards, > Robert Fauck > Wellington hospital, NZ > > > This email or attachment(s) may contain confidential or legally privileged > information intended for the sole use of the addressee(s). Any use, > redistribution, disclosure, or reproduction of this message, except as > intended, is prohibited. If you received this email in error, please notify > the sender and remove all copies of the message, including any attachments. > Any views or opinions expressed in this email (unless otherwise stated) may > not represent those of Capital & Coast District Health Board. > > http://www.ccdhb.org.nz > > (1C_S1) > > > > ------------------------------ > > Message: 5 > Date: Tue, 11 Jun 2013 13:34:09 +0000 > From: "Jackman, Mary Ione" <ione.jack...@uconn.edu> > Subject: [Histonet] Heinz body stain on already dried blood smears. > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <b01edf48a11fde4baddb7e941aee85e7195b1...@maild.grove.ad.uconn.edu> > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > Does anyone have a protocol they like for demonstrating Heinz bodies on > already dried blood smears? There is no blood in tubes available any longer. > I saw a citation for using Wrights Giemsa, but is there one that is more > specific that anyone uses and is willing to share? > Thanks. > Ione Jackman > > > > > ------------------------------ > > Message: 6 > Date: Tue, 11 Jun 2013 09:08:40 -0500 > From: "Gloria Tharp" <gth...@pcasoutheast.com> > Subject: [Histonet] Telepathology > To: <Histonet@lists.utsouthwestern.edu> > Message-ID: <006701ce66ad$2c971fc0$85c55f40$@com> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have any recommendations for scanning equipment to have slides > read off site from main lab. Thanks. > > > > > > Gloria Tharp, BA, HTL(ASCP) > > Director of Operations > > PCA Southeast > > gth...@pcasoutheast.com > > 931-490-1005 > > 800-388-1294 > > > > > > ------------------------------ > > Message: 7 > Date: Tue, 11 Jun 2013 08:28:20 -0600 > From: Elizabeth Chlipala <l...@premierlab.com> > Subject: RE: [Histonet] Telepathology > To: Gloria Tharp <gth...@pcasoutheast.com>, > "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <14E2C6176416974295479C64A11CB9AE016411AB4898@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > Gloria > > There are many systems out there that perform scanning, your choice is > dependent upon how you are going to be utilizing the instrument, how many > slides per day that you will need to scan, if you will be utilizing any image > analysis algorithms, etc. > > The DPA has lots information on its website - > https://digitalpathologyassociation.org/ > > They are also hosting a free webinar on July 10th (this is the 1st of 4) to > sign up just go to this link > https://digitalpathologyassociation.org/2013-dpa-webinar-series > The webinar is titled Real-World Examples of Practical > Consultative/Collaborative Digital Pathology Programs > Wednesday, July 10 | 2 PM EST > > They also have a yearly conference, this year its in San Antonio you would be > able to visit with many of the vendors that sell scanning systems - here is > the link for that > https://digitalpathologyassociation.org/pathology-visions-2013. > > Good Luck > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > l...@premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp > [gth...@pcasoutheast.com] > Sent: Tuesday, June 11, 2013 8:08 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Telepathology > > Does anyone have any recommendations for scanning equipment to have slides > read off site from main lab. Thanks. > > > > > > Gloria Tharp, BA, HTL(ASCP) > > Director of Operations > > PCA Southeast > > gth...@pcasoutheast.com > > 931-490-1005 > > 800-388-1294 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 115, Issue 11 > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
