Hello,
I need to paraffin process native sheep anterior cruciate ligament (ACL)
that has been fixed in 10% neutral buffered formalin for 7 days. I was
wondering if anyone with more expertise on this subject could guide me
with the best lengths of times in the alcohols, xylenes, and paraffin to
fix ACL. I tried a 12 hour program but the sections crumbled in the
middle and it appeared that the paraffin had not fully perfused the
ligament.
I have access to the following program, and can alter the lengths of the
steps for as long as desired:
Program:
70%
80%
95%
95%
100%
100%
Xylene
Xylene
Paraffin
Paraffin
Paraffin
Any advice is much appreciated.
Thanks for your time,
Liz
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
