Hello Histonet! I'm a long time reader first time poster. Does anyone have experience processing guinea pig tissues? I have been processing kidney and heart but it is consistently coming out mushy in the middle. The mouse tissue comes out fine even when processed on the same run. I had the tissue grossed in thinner (2.5mm) thinking that perhaps it was too thick but it didn't seem to help. Also, it has been fixed in 10%NBF for several days.
I was just wondering if anyone else had similar problems with guinea pig? I appreciate in advance any advice or tips. Here is the protocol: Formalin 1hr 70% etOH 1hr 95% 1hr 100% 30min 100% 1hr 100% 1hr 100%1hr Clearify 1hr Clearify 1hr Clearify 1hr Paraffin 1hr paraffin 1hr All under pressure and heat only on the paraffin. Thanks Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
