Are you referring to a whole heart? If that is the case I think the fixation of the tissue will be very poor because Karnovsky's penetrate very slowly. I do not think it will be worth the effort and materials to process this tissue. René J.
From: "Molinari, Betsy" <bmolin...@texasheart.org> To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Sent: Wednesday, June 19, 2013 1:32 PM Subject: [Histonet] Karnovsky's Hi, I received a heart that was fixed in 2% Karnovsky’s. I was told it was initially kept under refrigeration but then stored at RT. I do not know how long the heart has been stored at RT. By the look of the tissue I would say quite a while. Is this tissue compromised in any way? Should I remove it from the Karnovsky’s and store in in something else? This heart is to undergo paraffin processing. Thanks for any input. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030 832-355-6524 (lab) 832-355-6812 (fax) <http://www.texasheart.org> Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.org<mailto:bmolin...@texasheart.org> | www.texasheart.org<http://www.texasheart.org/> 6770 Bertner Ave., MC 1-283, Houston, TX 77030 [Texas Heart Institute]<https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html>[THI News] [THI on Facebook] <http://www.facebook.com/Texas.Heart.Institute> [THI on Flicker] <http://www.flickr.com/photos/texasheart/sets/> [THI on Google] <https://plus.google.com/u/0/118043615690351997044/posts> [THI on Pinterest] <http://pinterest.com/texasheartinst/> [THI on Twitter] <http://twitter.com/Texas_Heart> [THI on You Tube] <http://www.youtube.com/TexasHeartInstitute> Confidentiality Notice: This message may be confidential and/or privileged. If you are not the intended recipient you may not review, disseminate or copy this e-mail, its contents and/or any attachments. Please immediately notify the sender If you have received this e-mail in error and delete it from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet