Pedro, I have been using a superb method from Bob Skinner for all my decalcified bone work. Basically, I infiltrate with 50% TissuePrep from Fisher Scientific and 50% EM400 from Leica. I then embed in straight EM400. I personally feel that I get good infiltration and I never have had a problem with my bone cutting. (knocking twice on wood....LOL) I might also mention that I decalcify with 5% formic acid and clear after dehydration using methyl salicylate instead of xylenes. Jack
Jack L RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology > From: jgarfi...@lifecell.com > To: lou...@princeton.huntingdon.com; rjbu...@yahoo.com; lguern...@ucsd.edu; > histonet@lists.utsouthwestern.edu > Date: Wed, 19 Jun 2013 14:17:22 -0500 > Subject: RE: [Histonet] Mixing Paraffin Brands > CC: > > Pedro, > > I recommend Paraplast-Xtra. > > Regards, > Jackie > > Jacqueline D. Garfield | Manager, Histology > > Main 908.947.1100 Fax 908.947.1085 > Direct 908.947.1182 > Email jgarfield@ lifecell.com > www.lifcell.com > > LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876 > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louro, Pedro > Sent: Wednesday, June 19, 2013 2:33 PM > To: Rene J Buesa; Lucie Guernsey; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Mixing Paraffin Brands > > Just out of curiosity, what paraffin would people out there recommend using > for animal bone joints and turbinates? > We are currently using parapalst plus and was thinking of changing to a > "harder paraffin" > > Any thoughts??? > > Pedro L. > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, June 19, 2013 2:24 PM > To: Lucie Guernsey; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Mixing Paraffin Brands > > Each paraffin has some additives to improve either its penetration rate or > density to section. > Mixing different melting points (MP) paraffin will result in another paraffin > with an intermediate MP and the sectioning will be different. > Preparing the block with the mixture will probably cause so troubles while > sectioning. > Why don't you just use them separate? There is no good reason to mix them and > the two paraffins you mention are of good quality. > René J. > > From: Lucie Guernsey <lguern...@ucsd.edu> > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, June 19, 2013 2:01 PM > Subject: [Histonet] Mixing Paraffin Brands > > > Hi, > > Does anyone know if there's a reason why one shouldn't mix different brands > of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56 > C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point > 52 C). Will the different melting points be a problem? > > If we were to use the McCormick paraffin, the only place it may mix with > the Fisherbrand paraffin is in the blocks themselves (as we refill the > embedder). But I don't want to compromise the quality of our blocks just to > not waste the free paraffin. > > Or, another option could be that we use the McCormick in the processor and > the Fisherbrand in the embedder. Could that cause issues in the blocks as > the tissue would be infiltrated with one brand and embedded in another? > > Maybe I'm over-thinking this...... > > Many thanks! > Lucie > > Lucie Guernsey > UCSD > Dept. of Pathology > lguern...@ucsd.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ******************************************************************************************** > Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect > ******************************************************************************************** > > LEGAL NOTICE > This message is confidential and contains information which may be legally > privileged. 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