0.1% Trypsin + 0.1% Calcium Chloride in PBS for 10-20 minutes at 37C -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Thursday, June 27, 2013 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pepsin pre-digestion before immuno staining
We use trypsin and protease fairly often in our lab in conjunction with immuno staining, but we have not used pepsin. An investigator wants us to use pepsin, and we do have some, but would appreciate any recommendations regarding its use - concentration, time, temperature. Thanks Paul M. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
