I am interested in the answer as I use this same technique on my rodent
samples and have not had this issue.
On 7/2/2013 8:07 AM, Connolly, Brett M wro
Histonetters -
We had some rat tissues that were removed at necropsy and immediately fixed in
formalin for 48 hrs. Since the tissues could not be processed after the 48 hr
fixation they were transferred to 70% ETOH for about a week, then processed and
stained with H&E.
The H&E staining looks faded/washed out when compared to previous rat tissue
that was fixed for 48 hr and then processed and stained without the extended time
in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study
was that we were doing some IHC stains and wanted to keep the same fixation time as
the previous study.
I have extra unstained slides left over - any suggestions on how to get some better,
crisper H&E results? Has anyone observed the washed out H&E appearance from
tissues stored in 70% ETOH.
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803
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