So, I'm late to this response, but there are several ways you can avoid 
floaters.  We utilize the ThinPrep process for all body fluids, bronch washes, 
urines or other fluids we receive.  The nature of this type of prep is 
considered a method for reducing the potential for floaters.  You could also 
use a cytospin and the cytospin collection fluid contains PEG, which helps to 
the cells adhere to the slide.

We generally triage our body cavity fluids by taking a drop (after the material 
has been placed into a Preservcyt vial) and make a quick wet prep.  We add a 
few drops of toluedine blue to the drop, cover slip and do a rapid review for 
any positive appearing cells.  If found, we stain the body cavity fluid 
separately.  If not, it is batched with the other non-gyn specimens and 
stained.  We do separate out our direct smear prepped FNA's though as they have 
more of a tendency to shed material when it was not spread correctly, which 
resulted in thick areas. We generally know if the FNA is positive or not 
because we attend most of those procedures and provide a rapid interpretation.

All stains are filtered if a positive case is encountered.

Hope this helps to the conversation,


Joe W. Walker, Jr. MS, SCT(ASCP)CM
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
NEW EMAIL: joewal...@rrmc.org
http://www.rrmc.org/
 Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet 
Recognition® and the Governor's Award for Performance Excellence


-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Thursday, June 27, 2013 10:13 AM
To: McCabe, Sara; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cytology Staining

Body fluids are stained separately as they have a high potential for floating.  
FNA's , bronchs, thyroids, urine ect. Can all be stained together.   After a 
malignant case is reported all stains are filtered and the first alcohol before 
each stain is discarded.  The first alcohol after each stain is discarded and 
the others rotated up with a fresh one at the end.

-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara
Sent: Wednesday, June 26, 2013 3:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cytology Staining

I have a question for those of you that also may perform cytology specimens at 
your facility.  How do you prevent carryover from one case to another?  For 
example, we had obvious carryover from a pleural fluid onto an FNA of a thyroid 
case.  This has never happened here (to our knowledge).  Has anyone else every 
experienced this?  We spray fixed our slides with an alcohol/acetone based 
fixative before staining them.  Any advice would be appreciated!

Sara McCabe, HT(ASCP)
DuBois Regional Medical Center


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