So, I'm late to this response, but there are several ways you can avoid floaters. We utilize the ThinPrep process for all body fluids, bronch washes, urines or other fluids we receive. The nature of this type of prep is considered a method for reducing the potential for floaters. You could also use a cytospin and the cytospin collection fluid contains PEG, which helps to the cells adhere to the slide.
We generally triage our body cavity fluids by taking a drop (after the material has been placed into a Preservcyt vial) and make a quick wet prep. We add a few drops of toluedine blue to the drop, cover slip and do a rapid review for any positive appearing cells. If found, we stain the body cavity fluid separately. If not, it is batched with the other non-gyn specimens and stained. We do separate out our direct smear prepped FNA's though as they have more of a tendency to shed material when it was not spread correctly, which resulted in thick areas. We generally know if the FNA is positive or not because we attend most of those procedures and provide a rapid interpretation. All stains are filtered if a positive case is encountered. Hope this helps to the conversation, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewal...@rrmc.org http://www.rrmc.org/ Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition® and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, June 27, 2013 10:13 AM To: McCabe, Sara; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cytology Staining Body fluids are stained separately as they have a high potential for floating. FNA's , bronchs, thyroids, urine ect. Can all be stained together. After a malignant case is reported all stains are filtered and the first alcohol before each stain is discarded. The first alcohol after each stain is discarded and the others rotated up with a fresh one at the end. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara Sent: Wednesday, June 26, 2013 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology Staining I have a question for those of you that also may perform cytology specimens at your facility. How do you prevent carryover from one case to another? For example, we had obvious carryover from a pleural fluid onto an FNA of a thyroid case. This has never happened here (to our knowledge). Has anyone else every experienced this? We spray fixed our slides with an alcohol/acetone based fixative before staining them. Any advice would be appreciated! Sara McCabe, HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
