Orla I know this might sound silly but normal mouse bone marrow contains both fat and hemoatopoietic marrow, the areas that are fat will appear like round voids. Is that what you are seeing?
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Orla M Gallagher [o.m.gallag...@sheffield.ac.uk] Sent: Friday, July 12, 2013 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone decalcifying/processing problem Dear Histonetters, Has anyone ever seen large holes/vacuoles in the bone marrow of mouse long bones after decalcification and processing to wax? We've had this problem with a study which was fixed in 4% paraformaldehyde and decalcified at 4 degrees C over a 14 day period according to a routinely-used protocol involving changes of 15% EDTA/0.5% PFA in PBS pH 8.0, rinsed in PBS to remove EDTA before processing to paraffin wax. The marrow is filled with large holes, almost like balloons. I'm happy to send images to anyone who might be able to help! Best wishes, Orla -- ************************** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallag...@sheffield.ac.uk STOP: Do you really need to print this e-mail? BE GREEN: Keep it on the screen. Times Higher Education University of the Year Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet