Plastic or metal molds? Sent from my iPhone
On Aug 1, 2013, at 11:26 AM, Tim Wheelock <[email protected]> wrote: > Hi Everyone: > > When I paraffin embed brain tissue, I gentle push down on all regions > (especially the edges) of the specimen(s). > It seems that all of the areas of the specimen (or each of several specimens) > lie flat on the bottom of the mold. > This should ensure that I can quickly obtain a full face when trimming, with > a minimum loss of tissue. > > However, I have found that when I remove the solidified blocks from the mold, > parts of the specimen, especially the cortical edges of the tissue, have > lifted up from the bottom of the mold. > I either have to re-embed the tissue, or trim through more tissue than I > would like to obtain a full face for sectioning. > > Has anyone had this problem? > How do people avoid it? > > Thank you, > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
