IF using Alexa - conjugated secondaries ( indirect IF) should give you the same results as a std stABCpx-DAB detection, for any given dilution of primary Ab. However, if you are using enhanced DAB.....sure, the primary Ab will have to be less dilute when visualising with an indirect IF method. So, do one run with your primary at 1/5K, 1/10K, 1/20K ( on three sequential sections, of course) ...detecting with, for eg, Alexa anti Rabbit 594 at 1/1K.
Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
