We transfer eyes out of Mod Davidsons after 24 hours and process with the other soft tissues in formalin- why transfer to alcohol? We have beautiful eye sections. Jackie O'
Sent from my iPhone On Aug 8, 2013, at 3:12 PM, Bea DeBrosse-Serra <bdebrosse-se...@isisph.com> wrote: > Patsy, > > 18-24 hours in Davidson's followed by 70% ethanol prior to processing. Some > people also store the eyes in 10%NBF. I will attach a schedule for you off > line. > > Bea > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > pru...@ihctech.net > Sent: Thursday, August 08, 2013 1:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] processing eyes > > > Can someone direct me to a protocol for processing eyes which have been > injected with Davidson's fixative and are now in 10% NBF. We had an eye > expert at the U for years I was going to send my techs to but apparently she > has finally retired because we cannot get a hold of her. Where are you Mary > Jo? > > > We need insight on how to process non-human primate eyes? They have been > injected with Davidson's fixative and are now in 10%NBF. We need a specific > processing schedule, embedding and sectioning advice. > > > > Thank you, > > > > Patsy > > > > > > > > > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > > 40864 E. Arkansas Ave > > Bennett, CO 80102 > > H 303-644-4538 > > C 720-281-5406 > > <mailto:prueg...@hotmail.com> prueg...@hotmail.com > > <mailto:rueggihcconsultin...@outlook.com> rueggihcconsultin...@outlook.com > > > > > > > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
