Could I ask exactly what you are looking for in mouse femur and human tonsil the tonsil I assume is a control? For what? Whatever you are looking for is obviously in human tonsil. Are you positive it is in murine femur. I used to stain for 15-20 targets in EDTA decaled mouse femurs but my control was also a mouse.
Ray Research Scientist Comparative Medicine University of Washington ----- Original Message ----- From: "Colleen Forster" <cfors...@umn.edu> To: "Histonet" <histonet@lists.utsouthwestern.edu> Sent: Thursday, August 15, 2013 4:58:35 PM Subject: [Histonet] IHC after EDTA decalcifying Hello fellow histonetters, Can someone who has worked with decalcifyied bone tell me if EDTA interferes with IHC staining? I was under the impression it did not but cannot get staining in mouse femurs that I have decaled in EDTA. I have used both the steamer and the decloaker for retrieval and in the human tonsil the stain is great. Any suggestions...... Thanks in advance! Colleen Forster U of MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
