Ann, There are many tuberculocidal cleaners. Just search for it. If you are in a hospital the central supply will most likely have some in stock. We use PD-128 by Spartan Chemical
http://www.spartanchemical.com/products/product/101604 Here are some references for cryostat cleaning: Cryostat decontamination references: College of American Pathologists, Commentary on certification questionnaire: 1) Decontaminate regularly with 70% alcohol. 2) Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily 3) Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997 NCCLS site: http://www.nccls.org/ Decontamination for HIV Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986) Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV). Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer). Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994. Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given). For those who like to read a lot: APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each. Tim Morken UCSF Medical Center -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Friday, August 16, 2013 8:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat cleaning ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal. Can someone tell me what they use that meets the CAP criteria listed above. thankis, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
