Hi All,' Hoping someone can give me some insight on if this happens/ happened to them. We change all of our reagents on the H&E stainer on Friday( which means the reagents sit idle over the weekend.) We rarely have to come in on the weekends due to having to embed/cut/stain tissues. However, on Monday or in this case today (Tues because of the Holiday), we have to stir our Eosin before putting our first rack through for staining because the Pathologists have been complained that the Eosin is too light if we don't. But I've had one of them tell me that a second and subsequent rack of slides does'nt have this problem. The Pathologist wants to know if by the tissue sitting in Formalin longer over the weekend on the processor vs. only sitting a few extra hours on the overnight run, is the extra time in Formalin causing this problem? I told him that I did'nt think so, but that I would ask. I also thought that maybe in addition to stirring the Eosin, maybe running a rack of "blank" slides through a staining program before puttng the patient tissue through might help. What do you all think??
Thanks so much!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com ====================================="This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =====================================_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
