We spin the sample down, pour off supernatant. Resuspend in 5 drops thromboplastin and 4 drops human serum we receive from our phlebotomy lab. Let firm for 5 minutes and then fix in NBF. The agar suggestion probably works similarly if you can't get serum. On Sep 6, 2013 9:39 AM, "Dessoye, Michael J" < mjdess...@commonwealthhealth.net> wrote:
> We have had mixed results in the past with cell blocks, but currently we > are filtering the fluid through a biopsy bag and processing that. We > seem to be retaining more specimen with that method (we have used > sponges and lens paper in the past). > > Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General > Hospital | An Affiliate of Commonwealth Health | > mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, > PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 > > > -----Original Message----- > From: Ann Specian [mailto:thisis...@aol.com] > Sent: Thursday, September 05, 2013 12:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell Block Preparation > > > I am getting complaints in regard to "insufficient" cell blocks. We > currently spin, pour off the supernatant, retrieve the sediment and > process in lens paper. > > Does anyone have a more current technique which renders better > cellularity? > > Also, do you know which renders a better cell block: a fresh specimen, > a specimen fixed in Cytolyt or a specimen fixed in 10% NBF? > > Thanks, > Ann > > _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ > > This email and any files transmitted with it are confidential and > intended solely for the use of the individual or entity to whom > they are addressed. > If you have received this email in error please notify the > originator of the message. This footer also confirms that this > email message has been scanned for the presence of computer viruses. > > Any views expressed in this message are those of the individual > sender, except where the sender specifies and with authority, > states them to be the views of Commonwealth Health. > > Scanning of this message and addition of this footer is performed > by Websense Email Security software in conjunction with > virus detection software. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet