I'm having some QC issues lately with my gram stain. The gram positive bacteria on the control slide are only mostly gram positive. It seems positive in some areas and negative in others. It's not like only the higher portions are positive either, it's patchy. When I differentiate dipping the slide once is too little (negatives are still positive and lots of background staining with crystal violet) and two dips is too many (patchy positives). I don't know how to do one and a half dips. Has anyone else ever had this problem or any suggestions?
I'm using Poly Scientific's stain with crystal violet, gram's iodine, acetone:alcohol 1:1 for differentiation, and Safronin O for the counterstain. Thank you! Caitlin Hesketh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
