Forgive me if I'm missing something here but I'm under the impression that you can not stain for fat on paraffin section unless they have been rendered unsoluable via post fixation in something like osmium . The fat would be removed during the processing cycle in the alcohols and xylenes.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 [email protected] www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Rene J Buesa Sent: Thursday, September 12, 2013 2:33 PM To: Stella Mireles; [email protected] Subject: Re: [Histonet] Fat Stain on Paraffin Slide ? Sudan Black René J. ________________________________ From: Stella Mireles <[email protected]> To: "[email protected]" <[email protected]> Sent: Thursday, September 12, 2013 1:15 PM Subject: [Histonet] Fat Stain on Paraffin Slide ? Ok Experts - Please advise me on a stain for Fat which I can do on paraffin sections? Could you expand on your own experience with such an animal. Thanks to All Stella Houston, Tx _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
