Hello Everyone,
I am currently looking to bring a few new antibodies in
house; KI-67,P40, and PAX8. I have not worked with these antibodies yet and
wanted to get some opinions from everyone. For P40 and PAX8; are you using a
monoclonal or polyclonal? Also what company are you getting them from? My
hospital deals mainly with Dako, Biocare, and Cellmarque. We are a very small
facility that does HIER with a waterbath, and then manual IHC pipetting with
the old sequenza coverplate tiles. So usually we prefer concentrate for
antibodies not pre-dilute because of the longer expiration times, and of course
the cost. So I guess what I am asking is, what clone do you prefer. What
company do you get it from? How much does it cost? And have you ever run it on
a manual setup, if so how was it to work with?
Thanks for all the help in advance,
Natalie J. Nagy
Histology Supervisor
Holyoke Medical Center
nagy_nata...@holyokehealth.com
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