Hello Everyone,
                      I am currently looking to bring a few new antibodies in 
house; KI-67,P40, and PAX8. I have not worked with these antibodies yet and 
wanted to get some opinions from everyone. For P40 and PAX8; are you using a 
monoclonal or polyclonal? Also what company are you getting them from? My 
hospital deals mainly with Dako, Biocare, and Cellmarque.  We are a very small 
facility that does HIER with a waterbath, and then manual IHC pipetting with 
the old sequenza coverplate tiles. So usually we prefer concentrate for 
antibodies not pre-dilute because of the longer expiration times, and of course 
the cost. So I guess what I am asking is, what clone do you prefer. What 
company do you get it from? How much does it cost? And have you ever run it on 
a manual setup, if so how was it to work with?
 
Thanks for all the help in advance,
 
Natalie J. Nagy
Histology Supervisor
Holyoke Medical Center
 
nagy_nata...@holyokehealth.com


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