I've been doing this PSR stain for a few years now on various types of tissue sections of pretty much routine thickness (4-5 microns). I was asked today if the stain would penetrate a thicker section - like 40 or more microns. Even a vibratome section. The tissue is pig optic nerve and a small bit of surrounding tissue. When stained with PSR, the routine thickness sections were awesome.
Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu<mailto:algra...@email.arizona.edu> Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet