Dianova has an excellent antibody for CD31 in mice for FFPE tissue samples.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mesru T [turke...@gmail.com] Sent: Wednesday, September 18, 2013 4:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ZInc formalin Dear Histonetters, I do not think there is a good CD31 (PECAM-1) antibody that will work on FFPE mouse tissues. If you have one please let me know. We use CD34 rat mono (Abcam) and Meca-32 Rat mono (Dev Hybridoma Bank Iowa Univ.) to label endothelial cells of blood vessels in FFPE mouse tissues. There is a CD31 Rat mono from BD that will work of 10% NBF fixed, sucrose protected cryo embedded mouse tissues with antigen retrieval. The same CD31 Rat mono from BD will also work on Zinc Fixative from BD cat# 51-7538KZ ( it is a solution of ZnCl2 and maybe other zinc salts but no aldehyde) fixed paraffin embedded mouse tissues. The morphology of the section will be suboptimal due to this novel fixative. If anybody has any experince with this Zinc Fixative please would you share? Regards, Mesruh Turkekul mskcc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet