I would like to fix my muscle tissue samples in formalin after freezing in OCT. Does anyone have experience or could give me some suggestion? I would like to avoid artifacts...
Laura Avogaro Centre for Integrative Biology University of Trento Via delle Regole, 101 38123 Mattarello (TN) Italy > > We have had reasonable results based on rapid thawing of frozen samples in > room temperature buffer followed by formalin fixation or rapid freezing in > OCT. With such large samples, though, there are bound to be large ice crystals in the interior of the specimen regardless of the method used for > freezing. > > ________________________________________ > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] on behalf of Walter Benton [wben...@cua.md] > Sent: Tuesday, October 01, 2013 12:46 PM > To: Morken, Timothy; Histonet > Cc: jmitch...@neurology.wisc.edu > Subject: [Histonet] RE: thawing and refreezing muscle? > > This may help. Never used it when I processed fresh/frozen muscle specimens but have heard good things. > > http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf > > http://www.lifebloodmedical.com/ > > Walter Benton HT(ASCP)QIHC > Histology Supervisor > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > ChesapeakeUrology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > ________________________________________ > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [timothy.mor...@ucsfmedctr.org] > Sent: Tuesday, October 01, 2013 12:07 PM > To: Histonet > Cc: jmitch...@neurology.wisc.edu > Subject: [Histonet] thawing and refreezing muscle? > > Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen > muscle (centimeter thick) and that is what they want to try. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it > to the intended recipient, you are hereby notified that any dissemination, > distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the > transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet