Thanks to everyone for their muscle refreezing methods. We ended up being able to quickly split the samples and cut without thawing. But we will try some out for the future, just in case.
Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Hawkins, Hal K. [mailto:hhawk...@utmb.edu] Sent: Tuesday, October 01, 2013 1:35 PM To: Walter Benton; Morken, Timothy; Histonet Cc: jmitch...@neurology.wisc.edu Subject: RE: thawing and refreezing muscle? We have had reasonable results based on rapid thawing of frozen samples in room temperature buffer followed by formalin fixation or rapid freezing in OCT. With such large samples, though, there are bound to be large ice crystals in the interior of the specimen regardless of the method used for freezing. ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Walter Benton [wben...@cua.md] Sent: Tuesday, October 01, 2013 12:46 PM To: Morken, Timothy; Histonet Cc: jmitch...@neurology.wisc.edu Subject: [Histonet] RE: thawing and refreezing muscle? This may help. Never used it when I processed fresh/frozen muscle specimens but have heard good things. http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf http://www.lifebloodmedical.com/ Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Tuesday, October 01, 2013 12:07 PM To: Histonet Cc: jmitch...@neurology.wisc.edu Subject: [Histonet] thawing and refreezing muscle? Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen muscle (centimeter thick) and that is what they want to try. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
