Please see Freida Carson's Histotechnology-A self Instructional text (2nd 
edition pg 98, or 3rd edition pps 117-118).  There you will find that the 
correct answer is "A"- 37-40 % formaldehyde.  In the Notes section she explains 
"Many laboratories use acetone or alcohol as the fixative for frozen section; 
however, concentrated formaldehyde yields morphologic preservation more like 
that seen in permanent sections. Fixation in alcoholic formalin is also good"
 Since the question asks for "optiomally" fixed, the correct answer would be 
"A" and not "D"

hope this helps.


AmySue Ruppert,HT(ASCP) MB(ASCP)
Marshfield Labs,Histology 



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