We don't do in-situ PCR, but the principle is that with formalin-fixed
tissues
your amplified product is trapped in the protein matrix on the slide.
On 3:59, Sarah Dysart wrote:
Anyone out there do this? If so, during the PCR step you are amplifying your
gene of interest, where does the amplified product go? Each step of the PCR
(from how I am understanding this...I'm new to molecular biology protocols...)
separates the double stranded sequence then copies it, and this goes on and on
for 30-40 cycles...where does the product go? Does it just wash off? If not
how is it binding to the tissue?
Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
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