Richard, I wish we could eliminate them but not only do our negatives displaying some non-specific staining, seems to be tissue dependent, but our in-house QA people say we need to continue using them per the data sheets accompanying many of our antibodies. We use Ventana UltraView DAB detection, a multimer kit.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Cartun, Richard Sent: Wednesday, October 09, 2013 3:59 PM To: Histonet Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC.... My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?". I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax [email protected]<mailto:[email protected]> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
