I gross prostate needle biopsies every week.
I receive 12 samples but convert them in 6 paraffin blocks. I stain the Medial Samples of each level and each side with Eosin and the Lateral Samples with EA - 36 .Example: Base Lateral Right ( EA - 36 ), with Base Medial Right ( Eosin ).Base Lateral Left ( EA - 36 ), with Base Medial Left ( Eosin ) and so on. I wash the excess of colorant with 96 alcohol, wrap the samples of each sextant in one paper and put them in one cassette. Then I put the Cassette in the First Alcohol to avoid washing the Stains with aqueous formalin. It is very important to cut the cylinders so they do not exceed 5 mm. long. You avoid the coiling and facilitate the embedding. After all that it is very easy to see the colors in the paraffin block.It saves time and money and there is no errors if you are a tidy quiet person. Cesar RomeroBuenos AiresArgentina _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet