Our immunofluorescence protein antibody stains are automated, but not the routine histochemical stains used for muscle biopsies. Between the importance of maintaining pH levels, incubation temperatures (SDH,NADH,COX, AtPase's, Amyloid, MAD, Myophos, Acid Phos) and the need of a rotator plate (ORO). Really the only options for automation would be the H&E, Gomori Trichrome and PAS.
Sarah E. Lewis HT, ASCP Laboratory Coordinator The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 -----Original Message----- From: Nails, Felton [mailto:flna...@texaschildrens.org] Sent: Wednesday, November 13, 2013 5:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] neuropath stains Are the basic muscle and nerve biopsy stains being performed manually or has someone automated these stains? ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet