Dr Cartun- We've had similar experiences to Hugh's. In the histo group we maybe only have 15 validated freezers (all Revco's), but throughout our company we have hundreds. Over the past few years our Research Operations has moved to purchasing Sanyos because of the turnover rates of the new ultra low Revco's. We have no Sanyo's in our group yet, so I do not have any feedback on their performance. (I've always used uprights, so I'm not much use there either.) Best of luck!
Martha Lab Manager Gaithersburg, MD ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Message: 2 Date: Fri, 13 Dec 2013 11:41:15 -1000 From: Hugh Luk <hlu...@msn.com> Subject: [Histonet] Freezer for tissue storage To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "richard.car...@hhchealth.org" <richard.car...@hhchealth.org> Message-ID: <blu168-w478c44f289786b2a012431c3...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Dr. Cartun, Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred. My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future. We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)? Just my $0.02. (And $0.02 will not buy anything, anymore.) Hugh UHCC path shared resource manager Honolulu, HI > ------------------------------ > > Date: Thu, 12 Dec 2013 18:51:57 +0000 > From: "Cartun, Richard" <richard.car...@hhchealth.org> > Subject: [Histonet] Freezer for tissue storage > To: Histonet <histonet@lists.utsouthwestern.edu> > Message-ID: > <9215bd4b0ba1b44d962a71c758b68d2e018a0...@hhcexchmb05.hhcsystem.org> > Content-Type: text/plain; charset="iso-8859-1" > > I need a recommendation for a -80 degree C. freezer for storing tissue > specimens. Do you prefer upright vs. chest? Thanks. > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.car...@hhchealth.org<mailto:richard.car...@hhchealth.org> > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > ***************************************** ------------------------------ Message: 3 Date: Fri, 13 Dec 2013 21:57:20 +0000 From: "Truscott, Tom" <ttrus...@vetmed.wsu.edu> Subject: RE: [Histonet] Freezer for tissue storage To: Hugh Luk <hlu...@msn.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "richard.car...@hhchealth.org" <richard.car...@hhchealth.org> Message-ID: <9ef5279ebdfe6e4fb6605e8f183a00276af15...@cvm77.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" We have both upright and chest and both have their pros and cons. We have had trouble with electrical circuits which aren't matched to the load that these freezers can put on them. An electrician told us that it can put an tremendous strain on the freezer motor if the circuit isn't right and two or three freezers start up at the same time- leading to shortened life of the freezer. So- make sure a good electrician is involved in your freezer set-up. Tom T -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hugh Luk Sent: Friday, December 13, 2013 1:41 PM To: histonet@lists.utsouthwestern.edu; richard.car...@hhchealth.org Subject: [Histonet] Freezer for tissue storage Dr. Cartun, Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred. My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future. We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)? Just my $0.02. (And $0.02 will not buy anything, anymore.) Hugh UHCC path shared resource manager Honolulu, HI > ------------------------------ > > Date: Thu, 12 Dec 2013 18:51:57 +0000 > From: "Cartun, Richard" <richard.car...@hhchealth.org> > Subject: [Histonet] Freezer for tissue storage > To: Histonet <histonet@lists.utsouthwestern.edu> > Message-ID: > <9215bd4b0ba1b44d962a71c758b68d2e018a0...@hhcexchmb05.hhcsystem.org> > Content-Type: text/plain; charset="iso-8859-1" > > I need a recommendation for a -80 degree C. freezer for storing tissue > specimens. Do you prefer upright vs. chest? Thanks. > > > > Richard > > > > Richard W. Cartun, MS, PhD > > Director, Histology & Immunopathology > > Director, Biospecimen Collection Programs > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 Office > > (860) 545-2204 Fax > > richard.car...@hhchealth.org<mailto:richard.car...@hhchealth.org> > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Fried <levi.fr...@gmail.com> Subject: [Histonet] Staining on Alcohol Fixed Smears. To: histonet@lists.utsouthwestern.edu Message-ID: <CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2kv3gyebrbu0he3...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 121, Issue 19 ***************************************** To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet