We manually stain, but may switch from hematoxylin to Acid Blue 19 to increase contrast with the red chromogen preferred by the pathologists.
Zehntner et al 2008. Synergistic tissue counterstaining and image segmentation techniques for accurate, quantitative immunohistochemistry. Journal of Histochemistry and Cytochemistry 56(10):873-880. Tresa -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Susan Foreman Sent: Thursday, December 19, 2013 10:38 AM To: [email protected] Subject: [Histonet] Counterstain on Ventana Benchmark Ultra Merry Christmas to all those out there in Histoland! How are other labs handling the counterstain for IHC/ICC stained slides on the Ventana Benchmark Ultra? Has anyone figured out how to counterstain online without purchasing Hematoxylin and Bluing directly from Ventana? Any sort of prep kit ? Our slides are beautiful, just hoping to reduce our cost/test. Many Thanks, Susan Foreman, HT (ASCP) (865)661-4734 (865)584-1933 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
