I have been sectioning 28 years and, like everyone, completely disagree, at least with respect to animal tissue. You can "oversoak" some tissues like brain and liver, but you can also cut through the over soaked region just by cranking the wheel a little under the puffy stuff is gone. Also, the oversoaking occurs usually through neglect - leaving the blocks on wet ice for a long time. My two cents.
Phil Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deanna Leslie Sent: Sunday, January 05, 2014 4:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Soaking artifact Has anybody in histoland ever heard of this? I have been cutting tissue for 25 yrs and until recently I had never heard of this! I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice! Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler. I am not even sure what it is supposed to look like or what type of problems it causes. Thanks for listening! Deanna Leslie HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet