Hi Richard, Unfortunately we do not have a cut-off time for urgent transplant biopsies (its seems a kid's hospital is different). We microwave process kidney, lung and liver biopsies, about 30 minutes in total, though if we can get away with it we extend this by 30 minutes for more fixation, using 10%NBF and microwave. We use a Micromed TT.
We can then do this out of hours and weekends - cost a bit in overtime though, but hopefully it is worth it. The morphology is nearly always better than routine processing (using ethanol and xylene) and my pathologists prefer this for routine renal biopsies as well. There seems to be less leaching out of cytoplasmic substances compared to the routinely processed biopsies. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Friday, 10 January 2014 11:47 AM To: Histonet Subject: [Histonet] TAT for Transplant Biopsies For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing? Do any of you handle specimens at night, weekends, or holidays? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org<mailto:richard.car...@hhchealth.org> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet