Hi Gudrun: I recommend you to get "The Microwave tool book" by Login and Dvorak (1994) I am also sending you under separate cover an article I wrote on the subject. As to your questions, the practice of histology has concluded that: 1- the physical principle is that microwaves excite ("shake") all chemical molecules with electrical charge and, in consequence, that "shaking" produces heat. That is why paraffin and any "non-polar" molecule cannot be heated in a MW oven per se. 2- infiltration is faster because the heat is generated within the tissues, not by external convection 3- proteins (and antigens as proteins themselves) are not adversely affected by MW radiation (or so the say). 4- everybody using MW tissue processing claims that IHC procedures are not affected by the procedure. Having said all of the above I personally do not like MW processing; there are many ways of having fast processing with conventional tissue processors. René J.
________________________________ From: Gudrun Lang <gu.l...@gmx.at> To: Histonet@lists.utsouthwestern.edu Sent: Monday, January 13, 2014 1:56 PM Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet