For formaldehyde-fixed material the pH has to be lower than for methanol-fixed
blood or marrow smears (for which pH6.8 is best).
For your sections try pH 5.0 first. Go down in steps of 0.5 if too blue; up in
steps of 0.5 if too red. The colour scheme is not a varied as with a blood
film, but it's prettier by far than H&E.
John Kiernan
Anatomy, UWO
London, Canada
= = =
On 23/01/14, "Berger, Rebecca" <berge...@email.chop.edu> wrote:
> Hi all,
> I am trying to look at eosinophils and neutrophils in FFPE muscle sections.
> Does anyone have experience with the Wright stain or a Wright staining kit on
> these types of sections? Or possibly a suggestion for a different protocol to
> visualize these cells?
> Thanks!
> Becky
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>
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