I have had fungal contamination in the Fast Green counterstain from Thermo.
Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Tuesday, February 04, 2014 10:33 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Fungus contamination Judith, What counterstain are you using for each? If it is the light green, then that may be the culprit. Also check your baskets that you to deparaffinze, they may need to be cleaned. Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnma...@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 ------------------------------ Message: 8 Date: Tue, 4 Feb 2014 15:55:11 +0000 From: "Pardue, Judith" <judith_par...@memorial.org> Subject: [Histonet] Fungus contamination To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <f8593d93099a864a9730359fa6992a87475b6...@chiex016.chi.catholichealth.net> Content-Type: text/plain; charset="us-ascii" Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 9 Date: Tue, 4 Feb 2014 09:11:35 -0700 From: Elizabeth Chlipala <l...@premierlab.com> Subject: [Histonet] RE: Fungus contamination To: "Pardue, Judith" <judith_par...@memorial.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79E0529B@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Judith Its been years ago but I wrote a ASCP tech sample on this - I can't remember what solution it was but one of the solutions we used for the GMS stain actually grew fungus in it and we were getting staining on top of the tissue sections also. If you don't think it's one of your solutions I would culture your distilled water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ------------------------------ Message: 10 Date: Tue, 4 Feb 2014 16:21:32 +0000 From: "Truscott, Tom" <ttrus...@vetmed.wsu.edu> Subject: [Histonet] RE: Fungus contamination To: "Pardue, Judith" <judith_par...@memorial.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9ef5279ebdfe6e4fb6605e8f183a00276c8f6...@cvm77.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" Hi Judith, If you have made completely fresh solutions, even any stock reagents and still have the problem, then the fungus may be arriving from another source. I once had a problem of pollen landing on my water bath and showing up on the stains that would detect it. You may have fungus on other slides but only showing up in the PAS and GMS. Tom T -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Tuesday, February 04, 2014 7:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fungus contamination Having a problem with fungus on top of both the pas and gms stain. There are no solutions used on both test. Other than distilled water we can not figure it out. All solutions have been changed and clean containers used. Judith Gale Pardue Histology Supervisor judith_pardue@memorial .org This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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