I check pH every time I make a new batch(bottle) and then periodically there 
after. 

Timothy N. Higgins, HT (ASCP), QIHC
 

> Message: 10
> Date: Tue, 11 Feb 2014 16:23:22 +0000
> From: "Gupta, Nilesh" <ngup...@hfhs.org>
> Subject: [Histonet] Buffer pH monitoring
> To: "'histonet@lists.utsouthwestern.edu'"
>       <histonet@lists.utsouthwestern.edu>
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>       <f06daf0dbfc4ec4c97b930056a759d5e247bf...@rhwmbx15.corp.ds.hfhs.org>
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> I just want to get an idea of how often labs monitor the pH of the buffers 
> used in IHC labs. Do you pH for every bottleof a lot received prior to use, 
> single bottle of a lot only or do you have to pH every time you reconstitute 
> the buffer.
> 
> It is a CAP checklist question for AP & here is how the checklist question is 
> worded. It says when a new "batch" is prepared or received. Any input is 
> greatly appreciated. Thanks Nilesh Gupta, M.D, Henry Ford Hospital, Detroit, 
> MI
> 
> The pH of the buffers used in immunohistochemistry is routinely monitored.
> 
> NOTE:  pH must be tested when a new batch is prepared or received.
> 
> Evidence of Compliance:
> 
> ✓      Written procedure defining pH range for each buffer in use AND
> 
> ✓      Records of initial and subsequent QC on each buffer
> 
> 
> 
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