Dear Histology Experts,
I need your help. I am trying to stain undecalcified bone (adult mouse limb) with Alizarin Red S. I was thinking about two options: 1. Fixed frozen sections with Cryojane tape transfer system then staining the sections with Alizarin Red. 2. Alcohol fixed whole limb (whole mount preparation) stained with Alizarin Red then decalcified and processed for paraffin embedding/sectioning. I am not sure if the dye will survive decalcification and paraffin processing procedures. 3. I am trying to avoid doing plastic embedding unless there is no other option. Any insights are highly appreciated. Regards, Mesru _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
