Lester, Probably not in most cases but it depends upon what the antibody is, whether as a an antihuman ab it also cross reacts to the species you are interested in and what detection reagents you are using. For instance if you have determined a rabbit anti human antibody also cross reacts in mouse tissue, you could use a detection such as for the Leica Bond by leaving out the link (they call it the post primary but it is rabbit anti mouse IgG to link mouse antibodies to the goat anti rabbit labeled polymer second step), since your ab is rabbit it would link directly to the anti rab labeled polymer and the anti mouse link which would bind non specifically to the mouse endogenous Ig can be avoided. You would have to do a lot of research to first determine if the anti human rabbit antibody you want to use (it should not be made in a mouse) cross reacts to mouse tissue. The species you are wanting to label is also very important, this system would only work for anti human rab abs that also react in mouse, rat or any other species except rabbit and goat because those are the species the detection is made in or against. We have research versions of the Leica Bond instruments which allows us to manipulate the instrument and detection for these purposes including replacing the rab anti ms link with another link, I am not sure if the pure clinical versions of the Bond allow this. Cheers, Patsy
Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E. Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, March 26, 2014 2:20 PM To: Lester Raff MD; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: IHC on animals Lester I would not, first of all not all antibodies that work in human will cross react with a particular species of animal. Another thing to consider is that most human based detection systems consist of dual link reagents, meaning that they work on both mouse and rabbit primaries these detection system are not ideal for working with animals and depending upon the species that you are working with may cause some problems in with background staining due to the secondary antibody or polymer binding to endogenous IgG in the samples. You can initially pilot your protocol on a particular species to see what happens but I would not take the chance and run a bunch of slides thinking that the protocol that you have in place will work in the particular species of animal you need to stain. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Wednesday, March 26, 2014 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on animals Hi: Can IHC validated for humans be performed on animal tissues without any protocol changes? Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet