Merce There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall. We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study. We might try it again. We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam.
Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce Sent: Thursday, April 17, 2014 8:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] human cells in rat tissue Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Mercè Martí Gaudes Histology Unit ________________________________ P Abans d'imprimir aquest missatge, si us plau, comprova que és realment necessari. El medi ambient és cosa de tots. La informació continguda en aquest missatge i en qualsevol fitxer adjunt és confidencial, privada i d'ús exclusiu per al destinatari. Si no és la persona a la qual anava dirigida aquesta informació, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol còpia, divulgació, distribució o utilització noautoritzada d'aquest correu electrònic i dels seus adjunts està prohibida en virtut de la legislació vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La información contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta información, por favor, notifique inmediatamente el envio erróneo al remitente y borre el mensaje. Cualquier copia, divulgación, distribución o utilización no autorizada de este correo electrónico y de sus adjuntos está prohibida en virtud de la legislación vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
