Hello there! We are having some issues with our liver biopsies and can't seem to find any solutions. The biopsies are sent to us from IR already in formalin, we bake them in a 65* C oven for 30 minutes (to ensure fixation) and then we microwave process them. The microwave process is reagent alcohol, 5 mins, isopropanol, 5 mins, and finally paraffin, 2 min at 65* C and 5 min at 70* C. Worth noting: this is our procedure for all our biopsies - liver, heart, kidney, GI - and liver is the only one we are having problems with. The livers section well and don't appear to be over processed; however, without exception, they have a drying artifact on the outer edges. I have spoken to IR and they confirmed that they do not leave the biopsies out to dry for any amount of time. If they can't put them directly into formalin, the liver cores are held on saline soaked pads for no longer than a minute. I have seen suggestions about local anesthesia causing problems, but as we have no control over that and it's a necessary evil, I'd like to see if anyone has a suggestion to help minimize the artifact.
Thanks! Merry Amber Lewis, HTL Children's Medical Center Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
