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Begin forwarded message: > From: narjes baazaoui <baazaouinar...@gmail.com> > Date: May 14, 2014 at 1:35:04 PM CDT > To: histonet-ow...@lists.utsouthwestern.edu > Subject: Help in Dil labeling of brain sections > > Hello all, > Please I need some help in the lipophilic dil labeling of free floating brain > sections. The mice brain were fixed in 1.5% PFA by cardia perfusion, then > left one hour in the same fixative. I used the Dil labeling solution for a > 200 um thick sections, the dilution was 1:150 in PBS. The sections were > incubated for 48h in the Dil labeling solution then 48 in PBS in the cold > room in the shaker. The problem that I had a very high background and I could > not see even one spine. I need to stain only a few neurons so I can do a > reasonable quantification of the size and number of the spines. Does anybody > has an idea if in this case the solution or the crystal Dil is better and can > I get an optimized protocol for this kind of staining. > Thank you so much > All the best > Narjes > > -- > Narjes Baazaoui, PhD student > Institute of Basic research (IBR), > Laboratory of chemical neuropathology > 3476715743 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
