Hi Andi, I have a Google Doc of our procedure. I just sent you an invite to view it. If anyone else wants to see it, just let me know I'll share :-) I know the procedure calls for overnight in the LFB solution. It's for the best, the results are much better if you are patient. It is really important to not run the rehydration to water. End in 95% ETOH, the water will really kill the stain.
Good luck, Amos On Thu, May 15, 2014 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu>wrote: > Message: 7 > Date: Wed, 14 May 2014 19:23:58 +0000 > From: "Grantham, Andrea L - (algranth)" <algra...@email.arizona.edu> > Subject: Re: [Histonet] Luxol Fast Blue > To: Christina Kreutzer <christina.kreutze...@gmail.com> > Cc: "<histonet@lists.utsouthwestern.edu>" > <histonet@lists.utsouthwestern.edu> > Message-ID: <cabfb32d-027a-4ee0-b8e9-6cab3261e...@email.arizona.edu> > Content-Type: text/plain; charset="us-ascii" > > There are several procedures online. Just google Luxol fast blue staining > procedures. It is an easy stain to do. > > Andi > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet