Thanks Sandra! My next step was to try that. Dakshna
----- Original Message ----- From: "Sandra E. Esparza" <sespa...@seton.org> To: "Dakshnapriya Balasubbramanian" <dakshnapr...@neo.tamu.edu> Sent: Tuesday, May 20, 2014 10:34:25 AM Subject: RE: [Histonet] HELP- Cryosectioning FAT! You might want to dry Liquid Nitrogen on the face of the block before you cut it. Sandra Sandra Esparza HT (ASCP), QIHC Histotechnologist Mohs Austin Dermatologic Surgery Center 512-324-7468 x84027 sespa...@seton.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Dakshnapriya Sent: Tuesday, May 20, 2014 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP- Cryosectioning FAT! Hi all, I've been having problems with cryosectioning fatty tissue for a long time now. The section leaves a hole in the middle. I know this is probably a much discussed topic, but I've tried a lot of strategies with no luck. The tissue is of mouse origin and has micro-lesions buried inside fat. So the fat needs to be cut in order to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tried rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -80C. Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
