&nb= sp;WHen I took mine i did both parts. for the written carson
cover to cover= and the thick bancroft and stevens(sp?) 2 times cover
to cover.
Nick(Rocky) Madar= y, HT/HTL(ASCP)QIHC
Joni Madary, PhD(in life)
<= div style="border-top:1px solid #bcbcbc;margin:5px 0px;">
On 05/20/14, hist= onet-requ...@lists.utsouthwestern.edu wrote:
Send Histonet = mailing list submissions to
[1]histonet@lists.utsout= hwestern.edu
To subscribe or unsubscribe via the World Wide = Web, visit
[2]http://lists.utsouthwe= stern.edu/mailman/listinfo/histonet
or, via email, send a message = with subject or body 'help' to
[3]histonet-r= equ...@lists.utsouthwestern.edu
You can reach the person man= aging the list at
[4]histonet-ow...@lists.utso= uthwestern.edu
When replying, please edit your Subject line = so it is more specific
than "Re: Contents of Histonet digest...&q= uot;
Today's Topics:
1. HTL certification (C= ecy Stan)
2. RE: HTL certification (Esther C Peters)
3. pos= itive control indoleamine 2-3 dioxygenase
(Pathology-Histology S= r. Supervisor)
4. immunohistochemisty coding (Michael LaFriniere) 5. antibody vials
(Clare Thornton)
6. RE: antibody vials (Mu= rphy, Valerie)
7. HELP- Cryosectioning FAT! (Balasubbramanian, Daks= hnapriya)
8. new processor (anita)
9. RE: antibody vials (A= nne Murvosh)
10. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Da= kshnapriya)
11. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Dak= shnapriya)
----------------------------------------------- -----------------------
Message: 1
Date: Mon, 19 May 2014 1= 2:32:36 -0500
From: Cecy Stan <[5]cecysta...@gmail.com>
Subject: [Histonet] HTL certification
To: [6]histone= t...@lists.utsouthwestern.edu
Message-ID:
<CACh=0wgaaEfV
DFmsuB-Jw8OKo=LKUP[7]apgujjkoeagjazasx...@mail.gmail.com= >
Content-Type: text/plain; charset=UTF-8
Hello ev= eryone,
I'm starting to prepare for my HTL certification (I am v= ery nervous
and anxious but also very excited about this decision to g= o for it).
I was just curious to know how you guys prepared for = it, and how
long it
took for you to prepare before taking the test. Wi= ll 6 months
preparation
be enough? (I know that may depend on the indi= vidual; it's just that
I
had my Masters over 10 years ago and I haven'= t studied this much
since
then).
I have Freida L. Carson's = 3rd Edition book -- quite daunting to
memorize --
but the outline ASCP= has provided for study seems to be helpful. Do
you
have any other bo= ok/study aid suggestions?
Thank you in advance for your input an= d advice!
C.A.
------------------------------
Message: 2
Date: Mon, 19 May 2014 17:46:47 +0000
From: Esther= C Peters <[8]epete...@gmu.edu>
Subject: RE: [Histonet] HTL ce= rtification
To: Cecy Stan <[9]cecysta...@gmail.com>,
= "[10]histonet@lists.utsouthwestern.edu"
<[11]histonet@lists.utsouthwestern.edu>
Me= ssage-ID: <[12]1400521609188.54...@gmu.edu>
Content-= Type: text/plain; charset="iso-8859-1"
Hi C.A.,
I don't have the HT or HTL, but from my college teaching experience in
histology and histotechniques, I just wanted to caution you that
memoriz= ing is not what you should be doing. You need to understand
concepts, so th= at when you need to troubleshoot problems you will be
able to think through= things, rule some things out, and make sense of
the situation. I see this = all the time with my students, they forget
things they memorize, but then t= hey finally understand things and
can figure things out. One of the new tea= ching tools is having
students prepare "concept maps," to see the= relationships of topics
and terms, and these linkages will help you in the= long run. For
histology examples, see:
[13]http://www.biologycorner.com/anatomy/tissues/tissue_conce
pt_map_samples.html
I don't know of any concept maps for his= totechnology on the web, but
I am going to add this to my course next year! Esther
Esther C. Peters, Ph.D.
Assistant Profes= sor
Environmental Science & Policy
George Mason University
4400 University Drive, MS 5F2
Fairfax, VA 22030-4444
Office: D= avid King Hall, Room 3050
Phone: 703-993-3462
Fax: 703-993-1066e-mail: [14]epete...@gmu.edu
[15]https://bluprd0511.outlook.com/owa/redir.aspx?C
=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKr
rDjhw= Ovk.&URL=http%3a%2f%2fesp.gmu.edu
______________= __________________________
From: [16]histonet-bo= un...@lists.utsouthwestern.edu <[17]histonet-b
oun...@lists.utsouthwestern.edu> on behalf of Cecy Stan <[18]cec
ysta...@gmail.com>
Sent: Monday, May 19, 2014 1:32 PM
To: = [19]histonet@lists.utsouthwestern.edu
Subject: [Hist= onet] HTL certification
Hello everyone,
I'm starting = to prepare for my HTL certification (I am very nervous
and anxious but= also very excited about this decision to go for it).
I was just= curious to know how you guys prepared for it, and how long
it
took fo= r you to prepare before taking the test. Will 6 months
preparation
be = enough? (I know that may depend on the individual; it's just that
I
ha= d my Masters over 10 years ago and I haven't studied this much
since
t= hen).
I have Freida L. Carson's 3rd Edition book -- quite daunti= ng to
memorize --
but the outline ASCP has provided for study seems t= o be helpful. Do
you
have any other book/study aid suggestions?
<= br />Thank you in advance for your input and advice!
C.A.
_______= ________________________________________
Histonet mailing list
[20]Histonet@lists.utsouthwestern.edu
[21]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Messa= ge: 3
Date: Tue, 20 May 2014 12:09:28 +0000
From: "Pathology= -Histology Sr. Supervisor" <[22]hi...@skm.org.pk>
Subject:= [Histonet] positive control indoleamine 2-3 dioxygenase
To: "[23]histonet@lists.utsouthwestern.edu"
<[24]histonet@lists.utsouthwestern.edu>
Cc: "[25]tahseen0...@gmail.com" <[26]tahseen0...@gmail.com>Message-ID:
<BBFD4ECF5CBFAE4E936663D= 9eb7aa8e62b266...@exchmbx.skm.org.pk>
Content-Type: text/plain;= charset="us-ascii"
Hi All,
We are going to opt= imize indoleamine 2-3 dioxygenase (IDO1)
expression in breast cancer tissue= by immunohistochemistry on
formalin fixed paraffin sections.
I shall = be thankful if any one please let me know about the
"positive control= .
Regards
Muhammad Tahseen
Sr.Supervisor Histopathology
SKMCH&RC Lahore
Pakistan
-----------------------= -------
Message: 4
Date: Tue, 20 May 2014 13:03:55 +0000
From: Michael LaFriniere <[27]michael.lafrini...@ccplab.com= >
Subject: [Histonet] immunohistochemisty coding
To: "= ;'[28]sarah.dys...@stdavids.com'" <[29]Sarah.Dysart
@stdavids.com>,
"[30]histonet@lists.utsou= thwestern.edu"
<[31]histonet@lists.utsout= hwestern.edu>
Message-ID:
<4A2A16B9707CE04E9C[32]b6c82dc18c1d29670...@ahcmsasexch02.my.ahc.lo
cal>
Content-Type: text/plain; charset="us-ascii"
Hi Sarah,
Joyce is correct, under medicare changes in patho= logy coding, you
can not bill for each of the blocks of the same antibody a
pplications, only one charge per specific antibody mentioned in the
patholo= gy report is allowable. Medicare immuno billing is per
"specimen"= only for each specific antibody used, example if you used
s100 for the sam= e specimen (wide melanoma excision)on 15 different
blocks, under medicare y= ou can only bill one GO461, yes- not fair
when the need is appropriate,
I must voice my opinio)- this probably will lower some of the over
utilization and inappropriate billing practices that many of us have
seen = in the immunohistochemistry world and the labs that have
capitalized on the= practice, the problem with the lower
reimbursements and change in medicare= coding hurts many of our labs
who have used the utilization of immunohisto= chemistry appropriately.
However, "under current 2014&= quot; CPT coding on non medicare can
you bill for the additional work per b= lock using the 88342 and 88343
cpt code, I am sure moving forward we are go= ing to continue to see
adjustments to the pathology coding processes!
Michael R. LaFriniere, HT (ASCP)
Executive Director
Capital Choice Pathology Laboratory
12041 Bournefield Way, Suit= e A * Silver Spring, MD 20904
P: 240.471.3427 * F: 240.471.3401 * Ce= ll 410-940-8844
[33]michael.lafrini...@ccplab.com
-----Original Message-----
From: [34]histonet-boun...@lists.utsouthwestern.edu [ma
ilto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joy= ce K.
Sent: Monday, May 19, 2014 12:21 PM
To: '[35]Sarah.Dy= s...@stdavids.com';
[36]histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Billing
No - one G0461.
<= br />If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1
and G0462= x 2. j
Joyce Weems
Pathology Manager
678-843-7376 Pho= ne
678-843-7831 Fax
[37]joyce.weems@emoryhealthcare.o= rg
[38]www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-ma= il, including any attachments is the property of Saint
Joseph's Hospital an= d is intended for the sole use of the intended
recipient(s). It may contai= n information that is privileged and
confidential. Any unauthorized review= , use, disclosure, or
distribution is prohibited. If you are not the intend= ed recipient,
please delete this message, and reply to the sender regarding= the
error in a separate email.
-----Original Message-----
= From: [39]histonet-boun...@lists.utsouthwestern.edu
[[40]mailto:histonet-bounces@lists.utsouthwestern.e= du] On Behalf Of
[41]sarah.dys...@stdavids.com
Sent: Mon= day, May 19, 2014 12:00 PM
To: [42]histonet@lists.utsout= hwestern.edu
Subject: [Histonet] Billing
When it comes = to the medicare codes...question...
So you have one specimen tha= t has 10 blocks.
AE1/AE3 is ordered on all 10 blocks.
Can you bil= l AE1/AE3 1st Antibody once (G0461), then AE1/AE3
Additional (G0462) nine t= imes?
Thanks,
Sarah E. Dysart, BA, HT (ASCP), QIHC (A= SCP) Pathology Supervisor St.
David's North Austin Medical Center
1222= 1 North Mopac Expressway
Austin, Texas 78758
(512)901-1220
_______________________________________________
Histonet mailing= list
[43]Histonet@lists.utsouthwestern.edu
[44]http://lists.utsouthwestern.edu/mailman/list= info/histonet
________________________________
Th= is e-mail message (including any attachments) is for the sole use
of
t= he intended recipient(s) and may contain confidential and
privileged
i= nformation. If the reader of this message is not the intended
recipien= t, you are hereby notified that any dissemination,
distribution
or cop= ying of this message (including any attachments) is strictly
prohibite= d.
If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
or= iginal message (including attachments).
________________________= _______________________
Histonet mailing list
[45]H= isto...@lists.utsouthwestern.edu
[46]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<= br />
------------------------------
Message: 5
Date: = Tue, 20 May 2014 10:48:20 -0400
From: Clare Thornton <[47]CThor= n...@dahlchase.com>
Subject: [Histonet] antibody vials
To:= "[48]histonet@lists.utsouthwestern.edu"
= <[49]histonet@lists.utsouthwestern.edu>
Mes= sage-ID:
<C9D78FFC9D668B4CBEA4405F[50]84697 504013d73deb...@iris.dahlchase.net>
Content-Type: text/plain; c= harset="us-ascii"
Does anyone have any good ideas fo= r keeping the little vials of
concentrated antibodies neat and organized in= the fridge? Right now
we keep ours in empty pipette tip boxes (without ti= p holders) but
they're always falling over and mixed up. We use several di= fferent
vendors for our concentrated antibodies, so our vials are all diffe rent
sizes. Thanks for any ideas!
Clare J. Thornton= , HTL(ASCP)QIHC
Lead Immunohistochemistry Technologist
Dahl-Chase= Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401 [51]cthorn...@dahlchase.com<[52]mailto:cthornton@dahl=
chase.com>
----------------------------= --
Message: 6
Date: Tue, 20 May 2014 14:59:21 +0000
Fr= om: "Murphy, Valerie" <[53]murp...@karmanos.org>
= Subject: [Histonet] RE: antibody vials
To: Clare Thornton <[54]= cthorn...@dahlchase.com>,
"histonet@l= ists.utsouthwestern.edu"
<[55]histonet@li= sts.utsouthwestern.edu>
Message-ID:
<A0CD799E6FCD494= 9AAD4[56]a25377936712ae1bb...@ex-mb2.kci-net.k
armanos.org>
Content-Type: text/plain; charset="us-ascii= "
I use cardboard cryovial boxes and they work quite well. = You can
remove some of the dividers to make space for the larger vials.
[57]http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Fre
eze= r-Racks/Cryovial-Boxes-Cardboard/
Valerie = Ratliff B.Sc HTL(ASCP)
Research Assistant
Department= of Oncology
Karmanos Cancer Institute
4100 John R
Detroit, MI 48201
Telephone: (313) 576 8282 Fax: (313) 576 8306
E-mail: [58]murp...@karmanos.or= g
Better treatments. Better outcomes.
________________________________________
From: [59]histonet-boun...@lists.utsouthwestern.edu
[[60]histonet-boun...@lists.utsouthwestern.edu] on behalf of Clare
Thornto= n [[61]cthorn...@dahlchase.com]
Sent: Tuesday, May 20, 2014 1= 0:48 AM
To: [62]histonet@lists.utsouthwestern.edu
Subject: [Histonet] antibody vials
Does anyone have any good = ideas for keeping the little vials of
concentrated antibodies neat and orga= nized in the fridge? Right now
we keep ours in empty pipette tip boxes (wi= thout tip holders) but
they're always falling over and mixed up. We use se= veral different
vendors for our concentrated antibodies, so our vials are a= ll
different sizes. Thanks for any ideas!
Clare J. = Thornton, HTL(ASCP)QIHC
Lead Immunohistochemistry Technologist
Da= hl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, = ME 04401
[63]cthorn...@dahlchase.com<[64]mailto:cthornto= n...@dahlchase.com>
__________________________________= _____________
Histonet mailing list
[65]Histonet@li= sts.utsouthwestern.edu
[66]http://li= sts.utsouthwestern.edu/mailman/listinfo/histonet
----------- Confidentiality Notice: This email message, including any
attachments , is for the sole use of the intended recipient(s) and may
contain
confiden= tial and/or privileged information. If you are not the
intended recipient(s= ), you are hereby notified that any
dissemination, unauthorized review, use= , disclosure or distribution
of this email and any materials contained in a= ny attachments is
prohibited. If you receive this message in error, or are = not the
intended recipient(s), please immediately notify the sender by emai= l
and destroy all copies of the original message, including attachments.
------------------------------
Message: 7Date: Tue, 20 May 2014 10:23:27 -0500 (CDT)
From: "Balasubbra= manian, Dakshnapriya"
<[67]dakshnapr...@neo.tamu.edu><= br />Subject: [Histonet] HELP-
Cryosectioning FAT!
To: [68]histonet@lists.utsouthwestern.edu
Message-ID:
<21128= 5224.17352091.1400599[69]407431.javamail.r...@neo.tamu.edu>
Content-Type: text/plain; charset=utf-8
Hi all,
I've been having problems with cryosectioning fatty tissue for a lo ng
time now. The section leaves a hole in the middle. I know this is
probab= ly a much discussed topic, but I've tried a lot of strategies
with no luck.= The tissue is of mouse origin and has micro-lesions
buried inside fat. So = the fat needs to be cut in order to get to the
lesion. I started with a tem= perature of -17C and tried upto -25C and
also at -50C; didn't work at any o= f these temperatures. I tried
rubbing the block, blade and anti-roll plate = with dry ice-again,no
luck.
I rapid-freeze the tissue with OCT = in LN2 and then store the block
at -80C. Could I try sectioning the block d= irectly from -80C? (never
done that).
Any other suggestions on = how to tackle this?
Any advice is much appreciated!!
= Thanks,
Dakshna
------------------------------<= br />
Message: 8
Date: Tue, 20 May 2014 10:26:13 -0500
From:= anita <[70]azdud...@hotmail.com>
Subject: [Histonet] new p= rocessor
To: "[71]Histonet@lists.utsouthwestern.edu= "
<[72]histonet@lists.utsouthwestern.edu<= /a>>
Message-ID: <COL131-W4520B92[73]279A33E8D42CCF0D= 1...@phx.gbl>
Content-Type: text/plain; charset="iso-885= 9-1"
Thanks everyone for your input on processors, I think= we will stay
with the VIP, it has been a good machine just getting older n= ow.
everyone have a great day!!!!
Anita Dudley
Providence Hosp
Mobile, Alabama 3= 6695
------------------------------<= br />
Message: 9
Date: Tue, 20 May 2014 08:29:05 -0700
From:= "Anne Murvosh" <[74]amurv...@advancederm.net>
Subject: RE: [Histonet] antibody vials
To: "Clare Thornton"= ; <[75]cthorn...@dahlchase.com>,
<[76]histonet@lists.utsouthwestern.edu>
Message-ID:
<4A= D6A4E531E8C943A7[77]30559B6B81DF07DA8970@dc.Advancederm= .net>
Content-Type: text/plain; charset="us-ascii"= ;
We got some plexiglass dividers at the "Container Store&q= uot; if you
have one
near or check online. Can't remember what they w= ere called but they
had
different sized square compartments that fit v= arious venders
antibodies.
Anne
-----Original Message-----<= br />From:
[78]histonet-bounces@lists.utsouthwestern.= edu
[[79]mailto:histonet-bounces@lists.utsout= hwestern.edu] On Behalf Of
Clare
Thornton
Sent: Tuesday, May = 20, 2014 7:48 AM
To: [80]histonet@lists.utsouthwestern.e= du
Subject: [Histonet] antibody vials
Does anyone have = any good ideas for keeping the little vials of
concentrated antibodies= neat and organized in the fridge? Right now
we
keep ours in empty pi= pette tip boxes (without tip holders) but
they're
always falling over = and mixed up. We use several different vendors
for
our concentrated a= ntibodies, so our vials are all different sizes.
Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC
Lead Immunohi= stochemistry Technologist
Dahl-Chase Diagnostic Services
417 Stat= e Street, Suite 540
Bangor, ME 04401
cthornton@dahlchase.= com<[81]mailto:cthorn...@dahlchase.com>
_= ______________________________________________
Histonet mailing list[82]Histonet@lists.utsouthwestern.edu
[83]http://lists.utsouthwestern.edu/mailman/listinfo/= histonet
------------------------------
Message: 10
Date: Tue, 20 May 2014 10:59:16 -0500 (CDT)
From: = "Balasubbramanian, Dakshnapriya" <dakshnapriya@neo.t= amu.edu>
Subject: Re: [Histonet] HELP- Cryosectioning FAT!
To: "Sandra E. Esparza" <[84]sespa...@seton.org>,
= [85]histonet@lists.utsouthwestern.edu
Message-ID: <601376475.17568918.1400601[86]556617.JavaMail.ro=
o...@neo.tamu.edu>
Content-Type: text/plain; charset=utf-8
Thanks Sandra! My next step was to try that.
Dakshna
----- Original Message -----
From: "Sandra E. Esparza"= ; <[87]sespa...@seton.org>
To: "Dakshnapriya Balasubbram= anian" <[88]dakshnapr...@neo.tamu.edu>
Sent: Tues= day, May 20, 2014 10:34:25 AM
Subject: RE: [Histonet] HELP- Cryosectio= ning FAT!
You might want to dry Liquid Nitrogen on the face of t= he block
before you cut it.
Sandra
Sandra Esparza H= T (ASCP), QIHC
Histotechnologist Mohs
Austin Dermatologic Surgery= Center
512-324-7468 x84027
[89]sespa...@seton.org
-----Original Message-----
From: [90]his= tonet-boun...@lists.utsouthwestern.edu [[91]mailto
:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Balasubbramani= an, Dakshnapriya
Sent: Tuesday, May 20, 2014 10:23 AM
To: [92]histonet@lists.utsouthwestern.edu
Subject: [Histonet] = HELP- Cryosectioning FAT!
Hi all,
I've been having pr= oblems with cryosectioning fatty tissue for a
long time now. The section le= aves a hole in the middle. I know this
is probably a much discussed topic, = but I've tried a lot of
strategies with no luck. The tissue is of mouse ori= gin and has
micro-lesions buried inside fat. So the fat needs to be cut in = order
to get to the lesion. I started with a temperature of -17C and tried upto
-25C and also at -50C; didn't work at any of these temperatures.
I tri= ed rubbing the block, blade and anti-roll plate with dry
ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then
store the b= lock
at -80C. Could I try sectioning the block directly from -80C? (never
d= one that).
Any other suggestions on how to tackle this?
Any advice is much appreciated!!
Thanks,
Dakshna
<= br />_______________________________________________
Histonet mailing = list
[93]Histonet@lists.utsouthwestern.edu
[94]http://lists.utsouthwestern.edu/mailman/listi= nfo/histonet
CONFIDENTIALITY NOTICE:
This email m= essage and any accompanying data or files is
confidential and may contain p= rivileged information intended only
for the named recipient(s). If you are = not the intended
recipient(s), you are hereby notified that the disseminati= on,
distribution, and or copying of this message is strictly prohibited.
If= you receive this message in error, or are not the named
recipient(s), plea= se notify the sender at the email address above,
delete this email from you= r computer, and destroy any copies in any
form immediately. Receipt by anyo= ne other than the named
recipient(s) is not a waiver of any attorney-client= , work product,
or other applicable privilege.
-----= -------------------------
Message: 11
Date: Tue, 20 May 201= 4 11:03:08 -0500 (CDT)
From: "Balasubbramanian, Dakshnapriya"= ;
<[95]dakshnapr...@neo.tamu.edu>
Subject: Re: [Histon= et] HELP- Cryosectioning FAT!
To: Laurie J King <[96]k= ing.lau...@marshfieldclinic.org>,
histonet= @lists.utsouthwestern.edu
Message-ID:
<262036458.175957= 65.1400601[97]788819.javamail.r...@neo.tamu.edu>
= Content-Type: text/plain; charset=utf-8
Hi Laurie,
= No, the tissue hasn't been fixed before freezing. My prof doesn't
prefer th= e method, but if nothing else works, we might give it a
try.
An= y suggested protocols for fixation?
Thanks!
Dakshna
----- Original Message -----
From: "Laurie J King" <<= a class="parsedEmail"
href="mailto:king.lau...@marshfieldclinic.org"; ta
rget="_blank">king.lau...@marshfieldclinic.org>
To: "Dak= shnapriya Balasubbramanian" <[98]dakshnapr...@neo.tamu.edu>
Sent: Tuesday, May 20, 2014 10:37:35 AM
Subject: RE: [Histo= net] HELP- Cryosectioning FAT!
Dakshna,
Has this tiss= ue been fixed before freezing by any chance?
laurie
-= ----Original Message-----
From: [99]histonet-bou= n...@lists.utsouthwestern.edu
[[100]mailto:histonet= -boun...@lists.utsouthwestern.edu] On Behalf Of
Balasubbramanian, Daksh= napriya
Sent: Tuesday, May 20, 2014 10:23 AM
To: [101]histonet@lists.utsouthwestern.edu
Subject: [Histonet] HELP- Cryo= sectioning FAT!
Hi all,
I've been having problems wit= h cryosectioning fatty tissue for a
long time now. The section leaves a hol= e in the middle. I know this
is probably a much discussed topic, but I've t= ried a lot of
strategies with no luck. The tissue is of mouse origin and ha= s
micro-lesions buried inside fat. So the fat needs to be cut in order
to g= et to the lesion. I started with a temperature of -17C and tried
upto -25C = and also at -50C; didn't work at any of these
temperatures. I tried rubbing= the block, blade and anti-roll plate
with dry ice-again,no luck.
I rapid-freeze the tissue with OCT in LN2 and then store the block at
-8= 0C. Could I try sectioning the block directly from -80C? (never
done that). Any other suggestions on how to tackle this?
Any ad= vice is much appreciated!!
Thanks,
Dakshna
_____= __________________________________________
Histonet mailing list
= [102]Histonet@lists.utsouthwestern.edu
[103]http://lists.utsouthwestern.edu/mailman/listinfo/histon= et
_________________________________________________________ _____________
The contents of this message may contain private, protec= ted and/or
privileged information. If you received this message in error, = you
should destroy the e-mail message and any attachments or copies, and
yo= u are prohibited from retaining, distributing, disclosing or using
any info= rmation contained within. Please contact the sender and
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End of Histonet Digest, Vol 126, Issue 20
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References
1. file://localhost/tmp/3D"mailto 2. 3D"http://lists.utsouthwest=/
3. file://localhost/tmp/3D"mai 4. 3D"mailto:histonet- 5.
file://localhost/tmp/3D"mai 6. 3D"mailto:histonet@lists.utsouthwestern.edu";
7. 3D"mailto:ApGujjkoEAgjAzA 8. 3D"mailto:epete...@gmu.edu";
9. 3D"mailto: 10. 3D"mailto:histonet@lists.utsouthwest 11.
3D"mailto:histonet@lists.utsouthweste 12. 3D"mailto:1400521609188.54980@g 13.
file://localhost/tmp/3D"htt 14. 3D"mailto:epete...@gmu.edu";
15. file://localhost/tmp/3D"http 16. file://localhost/tmp/3D"mail 17.
file://localhost/tmp/3D"mai 18. 3D"mailto:cecysta...@gmail.com";
19. 3D"mailto:histonet@lists.utsouthwestern.edu";
20. 3D"mailto:Histonet@lists.utsouthwestern.edu";
21. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet";
22. file://localhost/tmp/3D"mailto 23.
3D"mailto:histonet@lists.utsouthwestern.edu";
24. 3D"mailto:histonet@lists.utsouthwestern.edu";
25. 3D"mailto:tahseen0...@gmail.com";
26. file://localhost/tmp/3D"mail 27. 3D"mailto:Mic 28.
3D"mailto:sarah.dys...@stdavids.com";
29. 3D"mailto:sarah.dys...@stdavids.com";
30. file://localhost/tmp/3D"mailt 31. file://localhost/tmp/3D"mailto 32.
3D"mailto:b6c82dc18c1d29670...@ahcmsasexch02.my.ahc. 33.
3D"mailto:michael.lafrin 34.
3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
35. 3D"mailto:sarah.dys...@stdavids.com";
36. 3D"mailto:histonet@li 37. 3D"mailto:joyce 38. 3D"http://www.sa=/
39. 3D"mailto:histonet-bounces@lists.utsout 40.
3D"mailto:histonet-bounces@lists.utsouthwe 41. 3D"mailto:Sarah.Dysart@s 42.
file://localhost/tmp/3D"mailto 43. 3D"mailto:Histonet@lists.utsouthwe 44.
3D"http://lists.utsouthwestern.edu/mailman/listin 45.
3D"mailto:Histonet@lists.utsouthwestern.edu";
46. file://localhost/tmp/3D"h 47. 3D"mailto:cthorn...@dahlchase.com";
48. 3D"mailto:histonet@lists.utsouthwester 49.
3D"mailto:histonet@lists.utsouthwester 50.
="mailto:84697504013d73deb...@iris.dahlchase.net";
51. 3D"mailto:cthorn...@dahlchase.com";
52. ="mailto:cthorn...@dahlchase.com";
53. file://localhost/tmp/3D"mailto 54. 3D"mailto:cthorn...@dahlchase.com";
55. ="mailto:histonet@lists.utsouthwestern.edu";
56. 3D"mailto:a25377936712ae1bb...@ex-mb2.kc 57.
3D"http://labscientific.com/Cryogenic-Suppli 58.
3D"mailto:murp...@karmanos.org";
59. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
60. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu";
61. 3D"mailto:cthorn...@dahlchase.com";
62. 3D"mailto:histo...@lists.uts 63. 3D"mailto:cthornton@dahlchase.c 64.
3D"mailto:cthorn...@dahlchase.com";
65. ="mailto:Histonet@lists.utsouthwestern.edu";
66. 3D"http://list=/
67. 3D"mailto:daks 68. 3D"mailto:histonet@lists.utsouthwestern.edu";
69. 3D"mailto:407431.JavaMai 70. 3D"mailto:azdud...@hotmail.com";
71. 3D"mailto:Histonet@li 72. 3D"mailto:histonet@lis 73.
="mailto:279a33e8d42ccf0d1...@phx.gbl";
74. 3D"mailto:amurv 75. 3D"mailto:cthorn...@dahlchase.com";
76. 3D"mailto:histonet@lists.utsouthwestern.edu";
77. 3D"mailto:30559B6B81DF07DA897 78. 3D"mailto:histonet-bounces@lists.u
79. 3D"mailto:histonet-bounces@lists 80. 3D"mailto:histonet@ 81.
3D"mailto:cthorn...@dahlchase.com";
82. 3D"mailto:Histonet@lists.utsouthwestern.e 83.
3D"http://lists.utsouthwestern.edu/mailman/listinfo/hi 84.
file://localhost/tmp/3D"mai 85. 3D"mailto:histo...@lists.utsouthwestern.ed
86. file://localhost/tmp/3D"mai 87. 3D"mailto:sespa...@seton.org";
88. 3D"mailto:dakshnapr...@neo.ta 89. file://localhost/tmp/3D"mai 90.
="mailto:histonet-boun...@lists.utsouthwestern.edu";
91. file://localhost/tmp/3D 92. 3D"mailto:histonet@lists.utsouthwestern.edu";
93. 3D"mailto:Histonet@lists.utsouthwes 94.
3D"http://lists.utsouthwestern.edu/mailman/listinf 95.
3D"mailto:dakshnapr...@neo.tamu.edu";
96. 3D"mailto:king.lau...@marshfieldclinic.org";
97. 3D"mailto:788819.JavaMail.root@neo. 98. file://localhost/tmp/3D"mailt
99. file://localhost/tmp/3D"mailt 100. 3D"mailto:h 101.
3D"mailto:histonet@lists.utsouthwestern.edu";
102. 3D"mailto:Histonet@lists.utsouthwestern.edu";
103. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet 104.
3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"_______________________________________________
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