HI Eva, Goat anti-HA antibody from Abcam supposed to work on FFPE sections: http://www.abcam.com/ha-tag-antibody-ab94918.html Don't know working dilution. Rabbit antibody from Abcam should probably work as well - I tried them only on formalin-fixed cryo-sections: http://www.abcam.com/ha-tag-antibody-chip-grade-ab9110.html I used it in dilution 1:200 for immunofluorescence. Chicken anti-HA antibody from Aves (cat.#ET-HA100) should work as well: http://www.aveslab.com/products/epitope-tag-and-gfp-antibodies/anti-ha-hemagglutinin-antibodies/ Should work in dilution 1:500-1:1000 for immunofluorescence.
Anatoli Gleiberman, Ph.D. Director of Histopathology Buffalo Biolabs LLC 73 High Street Buffalo, NY 14203 Phone: 716-849-6810x354 e-mail: agleiber...@buffalobiolabs.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Wednesday, May 21, 2014 9:54 AM To: histonet Subject: [Histonet] HA-tag antibody Hello, I am looking for an HA-tag antibody to detect a protein labeled with HA in formalin fixed paraffin embedded tissues. Could anyone suggest a published antibody? The tissue of interest is mouse so I would prefer an antibody made in another species. I have found several antibodies but can't find them published. Thank you, Eva Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
