Hi Mike, Edit the 'dummy' run and make station 3 with '0' time. It is time based and has a safety mechanism. That's why it pumps the previous station. Then test again.
A couple things may be an issue and need to be tested. 1. A hole, leak in the line of station 3. 2. Volume in reservoir bottle is too high 3. Kink in tube 4. Valve 'sticking'. Do the verify 7,8 test to hear the valve click in and out. A drop of xylene will fix this. Do this under supervision. 5. Check valve pressure and vacuum. View from behind and see the guage. 6. Clog in line 3. Do a hot water flush. 2-3 times. Regards Mitchell Wan 0418 745 750 P.O. BOX 2200, Runcorn, Brisbane, QLD 4113 -------- Original message -------- From: histonet-requ...@lists.utsouthwestern.edu Date:08/06/2014 3:03 AM (GMT+10:00) To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 127, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Processor problem (Mike Tighe) 2. RE: Processor malfunction - tissues not submerged ~ 9 hours (Shirley A. Powell) 3. paraffin dispenser (Davis, Cassie) 4. Re: Processor malfunction - tissues not submerged ~ 9 hours (jsjurc...@comcast.net) 5. Re: Processor problem (Rene J Buesa) 6. RE: Processor problem (Denice Stiner) 7. VIP3000 reagent bottle (Mitchell Wan (Wanpost)) ---------------------------------------------------------------------- Message: 1 Date: Fri, 6 Jun 2014 17:52:27 +0000 From: Mike Tighe <mti...@trudeauinstitute.org> Subject: [Histonet] Processor problem To: "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Message-ID: <1402077076740.7...@trudeauinstitute.org> Content-Type: text/plain; charset="iso-8859-1" I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning. I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself. Has anybody had this problem? Thanks for any help! Mike ------------------------------ Message: 2 Date: Fri, 6 Jun 2014 13:53:07 -0400 From: "Shirley A. Powell" <powell...@mercer.edu> Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours To: "O'Donnell, Bill" <billodonn...@catholichealth.net>, "Conway, Carla" <cmcon...@usgs.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25BF92F5E81@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="utf-8" Oh where is the like button. :) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 06, 2014 12:22 PM To: Conway, Carla; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Not since 1978. (Sorry - couldn't resist - Happy Friday!) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Friday, June 06, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=%2BfCU3trcgbZTxcTVVwV9PBYwA4sdkdeS3%2B8QsbqJBIg%3D%0A&s=f70156bac2d9c01ccd25a40b1c3fd9f124a0fe93c1fba0c52d4e1ef48da7a09e This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ------------------------------ Message: 3 Date: Fri, 6 Jun 2014 14:31:45 -0400 From: "Davis, Cassie" <cda...@che-east.org> Subject: [Histonet] paraffin dispenser To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <be7af02009c3a74f9b8344e70f3655cb0ab48ef...@chexcms01.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I recommend a slide incubator and a metal cup or two. The paraffin dispenser we purchased seem to be a relicensed commercial coffee pot. The spigot keeps clogging because they did not design it in a way that it would stay warm so, the paraffin cools in it and clogs. Cassie D. Michele" <mmargio...@bmhmc.org> Subject: [Histonet] paraffin dispenser Message: 1 Date: Fri, 6 Jun 2014 16:04:55 +0000 From: "Margiotta-Watz, Michele" <mmargio...@bmhmc.org> Subject: [Histonet] paraffin dispenser To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <230d0b9ec57d7a45a7a186c6ab4c7abc2a5e2...@bmh-exchange-01.bmhmc.org> Content-Type: text/plain; charset="us-ascii" Hi All, Anyone have a good recommendation for a reliable paraffin dispenser? The spigot keeps clogging on the one we have now. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Rd. Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 4 Date: Fri, 6 Jun 2014 18:43:13 +0000 (UTC) From: jsjurc...@comcast.net Subject: Re: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours To: "Shirley A. Powell" <powell...@mercer.edu> Cc: histonet@lists.utsouthwestern.edu Message-ID: <2129120167.2182515.1402080193013.javamail.r...@comcast.net> Content-Type: text/plain; charset=utf-8 Yet another reason to go microwave. This can't happen. ----- Original Message ----- From: "Shirley A. Powell" <powell...@mercer.edu> To: "Bill O'Donnell" <billodonn...@catholichealth.net>, "Carla Conway" <cmcon...@usgs.gov>, histonet@lists.utsouthwestern.edu Sent: Friday, June 6, 2014 12:53:07 PM Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Oh where is the like button. :) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Friday, June 06, 2014 12:22 PM To: Conway, Carla; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Not since 1978. (Sorry - couldn't resist - Happy Friday!) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Friday, June 06, 2014 9:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor malfunction - tissues not submerged ~ 9 hours Hello everyone, When it is working correctly, our tissue processor moves a basket of tissues through 10 reagent containers. Last night it malfunctioned and suspended the tissues above the 80% ethanol container. The tissues were high and dry for ~ 9 hours (!) until I placed them into 70% ethanol this morning. I will process them next week. Has this happened to anyone else and what tissue artifacts can I expect? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonet&k=NZz1SMMNIbmFChmIgSwv1Q%3D%3D%0A&r=E%2BUK3UdeD4AVR4ePVyCdWPXID5qqJ6mYOXk2vk%2FO6HA%3D%0A&m=%2BfCU3trcgbZTxcTVVwV9PBYwA4sdkdeS3%2B8QsbqJBIg%3D%0A&s=f70156bac2d9c01ccd25a40b1c3fd9f124a0fe93c1fba0c52d4e1ef48da7a09e This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 6 Jun 2014 13:56:15 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Processor problem To: Mike Tighe <mti...@trudeauinstitute.org>, "histonet@lists.utsouthwestern.edu \(histonet@lists.utsouthwestern.edu\)" <histonet@lists.utsouthwestern.edu> Message-ID: <1402088175.66031.yahoomail...@web120405.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 My advise is to call Sakura and schedule a maintenance visit. The rotary valve may be "acting up". Ren� J. On Friday, June 6, 2014 1:53 PM, Mike Tighe <mti...@trudeauinstitute.org> wrote: I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning. I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself. Has anybody had this problem? Thanks for any help! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 6 Jun 2014 14:19:53 -0700 From: Denice Stiner <denice.sti...@nahealth.com> Subject: RE: [Histonet] Processor problem To: Rene J Buesa <rjbu...@yahoo.com>, Mike Tighe <mti...@trudeauinstitute.org>, "histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Message-ID: <bddab7ea6ec20647937151d5fcfdfaa72d0b74b...@fmexmbx02.nah1.com> Content-Type: text/plain; charset="iso-8859-1" I do not have a Tissue Tek 3000 but it sounds like maybe there is a sensor that needs wiped clean. Clearly it's not ready the fluid level in station 3 and returning it back to 1 for safe keeping. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, June 06, 2014 1:56 PM To: Mike Tighe; histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Processor problem My advise is to call Sakura and schedule a maintenance visit. The rotary valve may be "acting up". Ren� J. On Friday, June 6, 2014 1:53 PM, Mike Tighe <mti...@trudeauinstitute.org> wrote: I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning. I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself. Has anybody had this problem? Thanks for any help! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Sat, 07 Jun 2014 18:21:43 +1000 From: "Mitchell Wan (Wanpost)" <mitch...@wanpost.com> Subject: [Histonet] VIP3000 reagent bottle To: HistoNET <histonet@lists.utsouthwestern.edu> Message-ID: <nk5n0emi9ojqaj53ety2inf4.1402129262...@email.android.com> Content-Type: text/plain; charset=utf-8 Hi All, Does anyone know where I can purchase some spare vip3000 reagent bottles? Regards Mitchell Wan 0418 745 750 P.O. BOX 2200, Runcorn,�� Brisbane, QLD 4113 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 127, Issue 10 *****************************************
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