I have another of those unusual projects and I'm looking for some advice of handling the drosophila flies before freezing (to fix or not to fix) and then freezing tips. Anybody have any experience with these flies?
They brought me some this morning but the results were not good. They killed the flies in 95% ETOH and strung them on a "fly collar" and put them in OCT and froze with a dry ice-alcohol slurry. Looks like ice crystals and a lot of shrinking and shattering under the scope. Andi G Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edu<mailto:algra...@email.arizona.edu> Tel: 520.626.4415 Fax: 520.626.2097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
